Advertisement

Chromatographia

, Volume 32, Issue 9–10, pp 436–440 | Cite as

Retention behavior of some monobasic penicillins and their quaternary ammonium ion pairs in reversed-phase high-performance liquid chromatography

  • D. J. Fletouris
  • J. E. Psomas
  • A. J. Mantis
Originals

Summary

The retention behavior of some monobasic penicillins (penicillin G, penicillin V, oxacillin, cloxacillin and dicloxacillin) and their quaternary alkylammonium ion pairs has been studied in a reversed-phase high-performance liquid chromatographic system using Partisil 5 μm ODS-3 asstationary phase and mixtures of acetonitrile and aqueous phosphate buffers as mobile phases. The influence of pH, pairing-ion chain length and concentration, and column temperature on retention has been investigated and the observed behavior of the compounds during the chromatographic process has been discussed.

Key Words

Column liquid chromatography Ion-pair chromatography Quaternary ammonium ion pairs Penicillins Retention behavior 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    I. Stephens, A. Grainger, J. Pharm. Pharmacol.,7, 702 (1955).Google Scholar
  2. [2]
    R. Thomas, Nature,191, 1161 (1961).Google Scholar
  3. [3]
    H. Hellberg, J. Assoc. Off. Anal. Chem.,51, 552 (1968).Google Scholar
  4. [4]
    J.E. Sinsheimer, D.D. Hong, J.H. Burckhalter, J. Pharm. Sci.,58, 1041 (1969).Google Scholar
  5. [5]
    D.V. Herbst, J. Food Prot.,45, 450 (1982).Google Scholar
  6. [6]
    W.A. Moats, J. Agric. Food Chem.,31, 1348 (1983).Google Scholar
  7. [7]
    A. Aboul Khier, G. Blaschke, M. El Sadek, Anal. Lett.,17, 1667 (1984).Google Scholar
  8. [8]
    S. Hendrickx, E. Roets, J. Hoogmartens, H. Vanderhaeghe, J. Chromatogr.,291, 211 (1984).CrossRefGoogle Scholar
  9. [9]
    S. P. Agarwal, J. Nwaiwu, J. Chromatogr.,323, 424 (1985).CrossRefGoogle Scholar
  10. [10]
    F. Kondo, J. Food Prot.,51, 786 (1988).Google Scholar
  11. [11]
    C. Hishta, D. L. Mays, M. Garofalo, Anal. Chem.,43, 1530 (1971).CrossRefGoogle Scholar
  12. [12]
    J. Hamann, A. Tolle, A. Bluthgen, W. Heeschen, Milchwissenschaft,30, 1 (1975).Google Scholar
  13. [13]
    T.A. Roy, S.S. Szinai, J. Chromatogr. Sci.,14, 580 (1976).Google Scholar
  14. [14]
    B. Meesschaert, P. Adriaens, H. Eyssen, J. Chromatogr.,136, 162 (1977).CrossRefGoogle Scholar
  15. [15]
    J.T. Rudrik, R.E. Bawdon, J. Liq. Chromatogr.,4, 1525 (1981).Google Scholar
  16. [16]
    V. Hartmann, M. Rodiger, Chromatographia9, 266 (1976).CrossRefGoogle Scholar
  17. [17]
    E.R. White, M.A. Carroll, J.E. Zarembo, J. Antibiot.,30, 811 (1977).Google Scholar
  18. [18]
    E.R. White, J.E. Zarembo, J. Antibiot.,34, 836 (1981).Google Scholar
  19. [19]
    T. Nakagawa, A. Shibukawa, T. Uno, J. Chromatogr.,239, 695 (1982).Google Scholar
  20. [20]
    W.A. Moats, J. Agric. Food Chem.,31, 880 (1983).Google Scholar
  21. [21]
    G.T. Briguglio, C.A. Lau-Cam, J. Assoc. Off. Anal. Chem.,67, 228 (1984).Google Scholar
  22. [22]
    A. Aboul Khier, G. Blaschke, M. El Sadek, Anal. Lett.,17, 1659 (1984).Google Scholar
  23. [23]
    U.R. Tjaden, H. Lingeman, R.A.M. Van der Hoeven, J.A.C. Bierman, H.J.E.M. Reeuwijk, J. Van der Greef, Chromatographia,24, 597 (1987).Google Scholar
  24. [24]
    C.T. Hung, J.K.C. Lim, A.R. Zoest, F.C. Lam, J. Chromatogr.,425, 331 (1988).Google Scholar
  25. [25]
    K. Tyczkowska, A.L. Aronson, J. Assoc. Off. Anal. Chem.,71, 773 (1988).Google Scholar
  26. [26]
    R.K. Munns, W. Shimoda, J.E. Roybal, C. Vieira, J. Assoc. Off. Anal. Chem.,68, 968 (1985).Google Scholar
  27. [27]
    K. Iwaki, N. Okumura, M. Yamazaki, J. Chromatogr.,504, 359 (1990).CrossRefGoogle Scholar
  28. [28]
    K. Berger, M. Petz, in: “Proc. Euro. Residue Conference on Residues of Veterinary Drugs in Food”,W.A.N. Haagsma, A. Ruiter andP.B. Czedik-Eysenberg, eds, Noordwijkerhout, Holland, 1990, p. 118.Google Scholar
  29. [29]
    M.C. Rouan, J. Chromatogr.,340, 361 (1985).Google Scholar
  30. [30]
    P.A. Ristuccia, J. Liq. Chromatogr.,10, 241 (1987).Google Scholar
  31. [31]
    M.A. Schwartz, J. Pharm. Sci.,54, 472 (1965).Google Scholar
  32. [32]
    J.P. Hou, J.W. Poole, J. Pharm. Sci.,60, 503 (1971).Google Scholar
  33. [33]
    J.M. Blaha, A.M. Knevel, D.P. Kessler, J.W. Mincy, S.L. Hem, J. Pharm. Sci.,65, 1165 (1976).Google Scholar
  34. [34]
    F. Salto, J.G. Prieto, M.T. Alemany, J. Pharm. Sci.,69, 501 (1980).Google Scholar
  35. [35]
    L.R. Snyder, J.J. Kirkland, “Introduction to Modern Liquid Chromatography”, 2nd ed., John Wiley & Sons, Chichester/New York, 1979, p. 468.Google Scholar
  36. [36]
    C. Horvath, W. Melander, I. Molnar, P. Molnar, Anal. Chem.,49, 2295 (1977).Google Scholar
  37. [37]
    B.A. Bidlingmeyer, J. Chromatogr. Sci.,18, 525 (1980).Google Scholar
  38. [38]
    J.H. Knox, R.A. Hartwick, J. Chromatogr.,204, 3 (1981).CrossRefGoogle Scholar
  39. [39]
    H.A.H. Billiet, A.C.J.H. Drouen, L. De Galan, J. Chromatogr.,316, 231 (1984).CrossRefGoogle Scholar
  40. [40]
    A. Tilly-Melin, Y. Askemark, K.-G. Wahlund, G. Schill, Anal. Chem.61, 976 (1979).Google Scholar
  41. [41]
    A.P. Goldberg, E. Nowakowska, P.E. Antle, L.R. Snyder, J. Chromatogr.,316, 241 (1984).CrossRefGoogle Scholar
  42. [42]
    R. Gloor, E.L. Johnson, J. Chromatogr. Sci.,15, 413 (1977).Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1991

Authors and Affiliations

  • D. J. Fletouris
    • 1
  • J. E. Psomas
    • 2
  • A. J. Mantis
    • 1
  1. 1.Laboratory of Milk and Milk ProductsSchool of Veterinary Medicine, Aristotle UniversityThessalonikiGreece
  2. 2.Laboratory of Food Hygiene, School of Veterinary MedicineAristotle UniversityThessalonikiGreece

Personalised recommendations