Zusammenfassung
Die Beeinflussung von Osteoblasten durch statische Magnetfelder wurde in vitro überprüft. Dazu wurden embryonale Osteoblasten von Rattenkalvarien in vitro dem Einfluß von Neodynium-Magneten (Intensität 178 mT) ausgesetzt. Als Marker für die Osteoblastenaktiyität diente die alkalische Phosphatase, die histochemisch nachgewiesen und semiquantitativ bestimmt wurde. Neben Kontrollgruppen mit entimagnetisierten Magneten (n=11) und ohne Magneten (n=9) wurden Magnetkonfigurationen mit anziehenden (n=11) und mit abstoßenden Magneten (n=10) verwendet. Es zeigte sich, daß die Form der Osteoblasten nicht von statischen Magneffeldern beeinflußt wird, ebenso orientieren sie sich nicht an den Feldlinien. Heterogene und homogene statische Magnetfelder haben ebenso wie eine unterschiedliche vertikale und horizontale Orientierung homogener Felder keine Wirkung auf die produzierte alkalische Phosphatase. Nach den Ergebnissen dieser In vitro-Studie war kein signitikanter Effekt statischer magnetischer Felder mit der Intensität von 178 mT auf Osteoblastenkulturen zu beobachten.
Summary
The aim of the present study was to investigate whether the influence of static magnetic fields can be demonstrated in an osteoblast cell culture. For this in-vitro study we used osteoblasts originating from the embryonal calyariae of rats. The total material (n=41) was divided in four group: a) cell cultures submitted to the fields of attracting magnets (n=11), b) cell cultures in the fields of repelling magnets (n=10), c) cell cultures under the influence of demagnetized magnets (sham group, n=11), d) control cultures (n=9). The cell cultures were subjected for 21 days to magnetie fields (intensity 178 ml) using Neodynium magnets. Osteoblast activity was histo chemically determined by staining the alkaline phosphatase produced. The quantitative evaluation was performed by measuring the optical density of the cell cultures and the results were statistically analysed. The results of these in-vitro study are 1. The optical density of the cell cultires was nearly the same in samples with or without the influence of magnetic fields. 2. No divergent reaction could be found between the application of homogeneous and heterogeneous magnetie fields. 3. The different orientation of homogeneous magnetic fields in vertical or horizontal direction did not effect the osteobfast cell culture. 4. The cellular distribution in the osteoblast cultures does not follow the orientation of the magnetic field lines. 5. The form of the osteoblasts was nearly the same among all four groups. According to our results a potential influence of static magnetic fields with an intensity of 178 mT on osteoblast activity could not be determined in the cell culture by evaluating the alkaline phosphatase production.
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Vortrag, gehalten auf der wissenschaftlichen Jahrestagung der Deutschen Gesellschaft für Kieferorthopädie 1990.
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Papadopulos, M.A., Hörler, I., Gerber, H. et al. Einfluß statischer magnetischer Felder auf die Aktivität von Osteoblasten: eine In-vitro-Untersuchung. Fortschritte der Kieferorthopädie 53, 218–222 (1992). https://doi.org/10.1007/BF02327638
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DOI: https://doi.org/10.1007/BF02327638