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Dexamethasone effects on vascular volume and tissue hematocrit in experimental RG-2 gliomas and adjacent brain

Summary

We have studied the effects of dexamethasone, a corticosteroid commonly used to treat brain tumors, on vascular volume and tissue hematocrit in RG-2 experimental rat gliomas.125I-RISA (radioiodinated serum albumin) was used to measure tissue plasma vascular volume (Vp) and51Cr labeled red cells were used to measure tissue red cell volume (Vrbc). Quantitative autoradiography was used to obtain local measurements of Vp and Vrbc in different brain and tumor regions. From these experimentally measured values, we calculated the tissue vascular volume (Vv), tissue hematocrit (THct) and systemic arterial hematocrit (AHct). The value reported primarily reflect capillary and small vessel volumes since blood drained from larger vessels during tissue processing and large vascular structures were avoided during analysis of the autoradiographic images. A total of 110 tumors were studied in 29 animals. There was a consistent trend for Vp and Vv to be reduced in all tumor regions after dexamethasone treatment, although a significant decrease was seen only in tumor center. Dexamethasone did not affect Vp or Vv in tumor-free brain regions. Dexamethasone appeared to have little effect on Vrbc in any brain or tumor region. THct was consistently, although not significantly, higher in tumors after treatment with dexamethasone; THct in tumor-free brain regions was unaffected by dexamethasone treatment.

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Abbreviations

AHct:

Arterial Hematocrit

Am :

Amount measured

BAT:

Brain Around Tumor

C:

Concentration in brain or tumor (Cb), plasma (Cp) or red blood cells (Crbc)

Hct:

systemic Hematocrit measured by centrifugation

RISA:

Radioiodinated Serum Albumin

THct:

Tissue Vascular Hematocrit

V:

Tissue Volume of plasma (Vp), red blood cells (Vrbc) or vascular space (Vv)

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Correspondence to Ronald R. Glasberg.

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Nakagawa, H., Groothuis, D.R., Owens, E.S. et al. Dexamethasone effects on vascular volume and tissue hematocrit in experimental RG-2 gliomas and adjacent brain. J Neuro-Oncol 6, 157–168 (1988). https://doi.org/10.1007/BF02327392

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Key words

  • brain tumors
  • vascular volume
  • dexamethasone
  • gliomas
  • tissue hematocrit
  • RISA