Tumor cord and growth in human brain tumors based on mathematical morphology
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The growth of human brain tumor was quantitatively investigated by using the anti-bromodeoxyuridine (BrdU) monoclonal antibody and the texture analyzing system (TAS). Tissues from thirty eight patients consisting of 22 gliomas, 8 brain metastases, 1 cerebellar hemangioblastoma and 7 normal white matters were applied in this study. Mean values of growth fractions (GFs) were 29.4% in group 1, 14.8% in group 2, and 6.5% in group 3. The higher value of GFs showed a significantly larger degree of perivascular aggregation of S-phase cells (DPAS). The mean number of cells in each tumor cord was approximately 20, which is not significantly larger than that of the normal group. But there was a significant difference between the non-selected area of the histologically malignant or benign gliomas and the proliferating area of the biologically malignant or benign brain tumors (t-value; p<0.001). A significant difference was also noticed in the mean area and diameter of tumor cords (t-value; p<0.001).
From the point of view of biological behavior in human brain tumor, it is suggested that one tumor cord contains approximately 20 viable cells in the proliferating area in both benign and malignant brain tumors, and that if viable cells increase to more than 20 cells in one TC, necrotic cells and the diameter and area of the TC will increase, because the vessel-density is decreased by the occlusion of the vessels and the death of the endothels.
Key wordshuman brain tumor anti-BrdU monoclonal antibody tumor cord texture analyzing computer system (TAS) mathematical morphology
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- 1.Baserga R: The cell cycle and cancer. Marcel Dekker, New York, 1971Google Scholar
- 4.Deane BR, Lantos PL: The vasculature of experimental brain tumours. (Part 1) A sequential light and electron microscope study of angiogenesis. J Neuroi Sci 49:55–66, 1981Google Scholar
- 10.Goz B: The effects of incorporation of 5-halogenated deoxyuridines into the DNA of eukaryotic cells. Pharmacol Rev 29:249–272, 1978Google Scholar
- 12.Hardman J: Angioarchitecture of gliomata. Brain 63:91–118, 1966Google Scholar
- 14.Hirst DG, Denekamp J: Tumour cell proliferation in relation to the vasculature. Cell Tissue Kinet 12:31–42, 1975Google Scholar
- 19.Howard A, Pelc SR: Synthesis of deoxyribonucleic acid in normal and irradiated cells and its relation to chromosome breakage. Heredity (suppl) 6:261–273, 1953Google Scholar
- 22.Kinsella TJ, Russo A, Mitchell JB, Rowland J, Jenkins J, Schwade J, Myers CE, Collins JM, Speyer J, Kornblith P, Smith B, Kufta C, Gladstein EA: A phase I study of intermittent intravenous bromodeoxyuridine (BUDR) with conventional fractionated irradiation. Int J Radiat Oncol Biol Phys 10:69–76, 1985Google Scholar
- 23.Kury G, Carter HD: Autoradiographic study of human nervous system tumors. Arch Path 80:38–42, 1965Google Scholar
- 26.Luthert PJ, Lantos PL: A morphometric study of the microvasculature of a rat glioma. Neuropath appli Neurobiol 11:461–473, 1985Google Scholar
- 29.Mitchell JB, Kinsella TS, Russo A, McPherson S, Rowland J, Kornblith P, Gladstein E: Radiosensitization of hematoprietic precursor cells (CFUc) in glioblastoma patients receiving intermittent intravenous infusions of bromodeoxyuridine (BUdR). Int J Radiat Oncol Biol Phys 9:457–463, 1983PubMedGoogle Scholar
- 31.Nagashima T, Hoshino T: Rapid detection of S-phase cells by anti-bromodeoxyuridine monoclonal antibody in 9L brain tumor cells in vitro and in situ. Acta Neuropathol (Berl) 66:12–17, 1985Google Scholar
- 32.Nagashima T, DeArmond SJ, Murovic J, Hoshino T: Immunocytochemical demonstration of S-phase cells by antibormodeoxyuridine monoclonal antibody in human brain tumor tissues. Acta Neuropathol (Berl) 67:155–159, 1985Google Scholar
- 36.Serra J: Image analysis and mathematical morphology. Academic Press, London New York Paris San Diego San Francisco Sāo Paulo Sydney Tokyo Toronto, 1982Google Scholar
- 37.Steel GG: Growth kinetics of tumours. Oxford, Oxford University Press, pp 76–78, 1977Google Scholar
- 43.Weller RO, Foy M, Cox S: The development and ultrastructure of the microvasculature in malignant gliomas. Neuropath appl Neurobiol 3:307–322, 1977Google Scholar
- 45.Yoshii Y, Maki Y, Tsuboi K, Tomono Y, Nakagawa K, Hoshino T: Estimation of growth fraction in human central nervous system tumors by bromodeoxyuridine. J Neurosurg 65:653–659, 1986Google Scholar