Plant and Soil

, Volume 116, Issue 1, pp 1–7 | Cite as

Restriction fragment length polymorphisms distinguish ectomycorrhizal fungi

  • John L. Armstrong
  • Nanci L. Fowles
  • Paul T. Rygiewicz
Article

Abstract

Basidiomycetous fungi, two saprophytes and three mycorrhizal, were used to assess the specificity of DNA hybridization for distinguishing genera from one another. Interspecific comparisons were done with several isolates of mycorrhizal fungi,Laccaria bicolor andL. laccata, collected from diverse geographical sites. The DNAs were digested with four restriction nucleases and separated by gel electrophoresis into patterns of DNA fragments called restriction fragment length polymorphisms (RFLPs). The RFLPs were hybridized with a radioactively-labeled DNA probe encoding Basidiomycetous ribosomal RNA genes. The five genera were discernable using both unprobed and probed RFLPs. Hybridization of probe DNA with RFLPs was isolate-specific for all nine Laccaria isolates examined. The reclassification of aL. bicolor isolate is supported, demonstrating that hybridization of RFLPs offers an additional tool for taxonomy of ectomycorrhizal fungi. The method may have field application for distinguishing known isolates if their DNA fingerprints are previously ascertained and are distinct from RFLPs of indigenous organisms.

Key words

ectomycorrhizal fungi endonuclease isolate identification RFLP restriction fragment length polymorphism rDNA 

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References

  1. An C S, Riggsby W S and Mullin B C 1985 Restriction pattern analysis of genomic DNA ofFrankia isolates. Plant and Soil 87, 43–48.CrossRefGoogle Scholar
  2. Bogler S A, Zarley C D, Burianek L L, Fuerst P A and Byers T J 1983 Interstrain mitochondrial DNA polymorphism detected in Acanthamoeba by restriction endonuclease analysis. Mol. Biochem. Parasitol. 8, 145–163.CrossRefPubMedGoogle Scholar
  3. Brown W M 1980 Polymorphism in mitochondrial DNA of humans as recorded by restriction endonuclease analysis. Proc. Natl. Acad. Sci. USA 77, 3605–3609.PubMedGoogle Scholar
  4. Cassidy J R, Moore D, Lu B C and Pukkila P J 1984 Unusual organization and lack of recombination in the ribosomal RNA genes ofCoprinus cinereus. Curr. Genet. 8, 607–613.Google Scholar
  5. Crameri R, Hintermann G and Hütter R 1983 Deoxyribonucleic acid restriction endonuclease fingerprint characterization of Ascomycete strains. Int. J. Syst. Bacteriol. 33, 652–655.Google Scholar
  6. Dobritsa S V 1985 Restriction analysis of theFrankia spp. genome. FEMS Microbiol. Ltrs. 29, 123–128.Google Scholar
  7. Fox G E, Stackebrandt E, Hespell R B, Gibson J, Maniloff J, Dyer T A, Wolfe R S, Balch W E, Tanner R S, Magrum L J, Zablen L B, Blakemore R, Gupta R, Bonen L, Lewis B J, Stahl D A, Luehrsen K R, Chen K N and Woese C R 1980 The phylogeny of prokaryotes. Science 209, 457–463.PubMedGoogle Scholar
  8. Garber R C and Yoder O C 1984 Mitochondrial DNA of the filamentous ascomyceteCochliobolus heterostrophus: Characterization of the mitochondrial chromosome and population genetics of a restriction enzyme polymorphism. Curr. Genet. 8, 621–628.CrossRefGoogle Scholar
  9. Harley J L and Smith S E 1983 Mycorrhizal Symbiosis. pp 483. Academic Press, New York.Google Scholar
  10. Hintermann G, Crameri R, Kieser T and Hütter R 1981 Restriction analysis of theStreptomyces glaucescens genome by agarose gel electrophoresis. Arch. Microbiol. 130, 218–222.CrossRefGoogle Scholar
  11. Hintz W E, Mohon M, Anderson J B and Horgen P A 1985 The mitochondrial DNAs of Agaricus: Heterogenecity inA. bitorquis and homogenecity inA. brunnescens. Curr. Genet. 9, 127–134.CrossRefGoogle Scholar
  12. Klich M A and Mullaney E J 1987 DNA restriction enzyme fragment polymorphism as a tool for rapid differentiation ofAspergillus flavus fromAspergillus oryzae. Exp. Mycology 11, 170–175.Google Scholar
  13. Malloch D, Castle A and Hintz W 1987 Further evidence forAgaricus brunnescens Peck as the preferred name for the cultivated Agaricus. Mycologia 79, 839–846.Google Scholar
  14. Maniatis T, Fritsch F E and Sambrook J 1982 Molecular Cloning: A Laboratory Manual. pp 545. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.Google Scholar
  15. Manicom B Q, Bar-Joseph M, Rosner A, Vigodsky-Haas H and Kotze J M 1987 Potential applications of random DNA probes and restriction fragment length polymorphisms in the taxonomy of the Fusaria. Phytopathology 77, 669–672.Google Scholar
  16. Marx D 1969 The influence of ectotrophic mycorrhizal fungi on the resistance of pine roots to pathogenic infections. I. Antagonism of mycorrhizal to root pathogenic fungi and soil bacteria. Phytopathology 59, 153–163.Google Scholar
  17. Murray M G and Thompson W F 1980 Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 8, 4321–4325.PubMedGoogle Scholar
  18. Noller H F 1984 Structure of ribosomal RNA. Annu. Rev. Biochem. 53, 119–162.CrossRefPubMedGoogle Scholar
  19. Sogin M L, Elwood H J and Gunderson J H 1986 Evolutionary diversity of eukaryotic small-subunit rRNA genes. Proc. Natl. Acad. Sci. USA. 83, 1383–1387.PubMedGoogle Scholar
  20. Southern E M 1975 Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503–517.PubMedGoogle Scholar
  21. Specht C A, Novotny C P and Ullrich R C 1984 Strain specific differences in ribosomal DNA from the fungusSchizophyllum commune. Curr. Genet. 8, 219–222.CrossRefGoogle Scholar
  22. Ullrich R C 1977 Natural distribution of incompatibility factors in Basidiomycetous fungi. Mycologia 69, 714–719.Google Scholar
  23. Vincent R D, Goewert R, Goldman W E, Kobayashi G S, Lambovitz A M and Medoff G 1986 Classification ofHistoplasma capsulatum isolates by restriction fragment polymorphisms. J. Bacteriol. 165, 813–818.PubMedGoogle Scholar
  24. Wu M M J, Cassidy J R and Pukkila P J 1983 Polymorphisms in DNA ofCoprinus cinereus. Curr. Genet. 7, 385–392.CrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • John L. Armstrong
    • 1
  • Nanci L. Fowles
    • 2
  • Paul T. Rygiewicz
    • 1
  1. 1.Corvallis Environmental Research LaboratoryUS Environmental Protection AgencyCorvallisUSA
  2. 2.NSICorvallisUSA

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