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On the role of divalent cations in the reaction mechanism of malic enzyme

Experientia volume 31, pages 1126–1128 (1975)Cite this article

Summary

The kinetic order of addition of Mg2+ andl-malate to malic enzyme has been determined. Mg2+ is the first to bind, and probably acts as a link between the substrate and the active site.

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Author information

Affiliations

  1. Departamento de Bioquímica, Facultad de Ciencias Bioquímicas, Universidad Nacional de Rosario, Suipacha 531, Rosario, (República Argentina)

    E. Massarini & J. J. Cazzulo

Authors
  1. E. Massarini
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  2. J. J. Cazzulo
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Additional information

This work was supported by grants from the Consejo Nacional de Investigaciones Cientificas y Técnicas de la República Argentina, and the Consejo de Investigaciones de la Universidad Nacional de Rosario. JJC and EM are members of the Carrera del Investigador Científico of the former and the latter institutions, respectively.

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Massarini, E., Cazzulo, J.J. On the role of divalent cations in the reaction mechanism of malic enzyme. Experientia 31, 1126–1128 (1975). https://doi.org/10.1007/BF02326748

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  • DOI: https://doi.org/10.1007/BF02326748

Keywords

  • Enzyme
  • Reaction Mechanism
  • Divalent Cation
  • Kinetic Order
  • Malic Enzyme