Summary
Thermospray liquid chromatography-mass spectrometry has been applied to the identification of by-products in precolumn derivatization by phenylisocyanate. These byproducts are eluted early in the same chromatographic region as the low molecular weight derivatives and were located by chromatographic analysis of a blank sample. Their identification would offer further qualitative information in the use of phenylisocyanate as a derivatizing agent. Five compounds resulted from the reaction of phenylisocyanate and the reaction medium were identified: two from a reaction between phenylisocyanate and methanol, two from the reaction between phenylisocyanate and water, and one from the polymerisation of phenylisocyanate.
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Rakotomanga, S., Baillet, A., Pellerin, F. et al. Identification of by-products in phenylisocyanate pre-column derivatization by thermospray liquid chromatography-mass spectrometry. Chromatographia 32, 125–129 (1991). https://doi.org/10.1007/BF02325014
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DOI: https://doi.org/10.1007/BF02325014