Summary
The DNP-amino acids have been divided into two groups depending upon their chromatographic behaviour on silicagel thin layers in different solvent systems. Five solvent systems were developed for each of the two groups. The systems reported provide rapid resolution and can separate mixtures of six to eight DNP-amino acids. The systems provide separations of DNP-ala/pro, leu/phe and leu/ile which are difficult to separate by one-dimensional TLC.
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References
R. Bhushan, R. N. Goyal, A. Agarwal, Biochem. Int.,11, 477 (1985).
R. Bhushan, N. Pant, J. Indian Chem. Soc.64, 197 (1987).
R. Bhushan, G. P. Reddy, Anal. Biochem.162, 427 (1987).
R. Bhushan, G. P. Reddy, F. Z. Anal. Chem.327, 798 (1987).
R. Bhushan, G. P. Reddy, Analyst112, 1467 (1987).
J. Rosmus, Z. Deyl, J. Chromatogr.,70, 221 (1972).
N. T. Cong, E. Tyihak, M. Vajda, E. Mincsouice, J. HRC and CC,5, 511 (1982).
L. Lepri, P. G. Desideri, D. Heimler, J. Chromatogr.,235, 411 (1982).
M. Brenner, A. Niederwieser, G. Pataki, in:E. Stahl (Editor), Thin-layer chromatography, a Laboratory Handbook, Academic Press, New York, p. 391 (1965).
J. M. Ghuysen, D. J. Tipper, J. L. Strominger, Methods Enzymol.,8, 685 (1966).
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Bhushan, R., Reddy, G.P. Some effective solvent systems for the rapid resolution of DNP-amino acids by thin-layer chromatography. Chromatographia 25, 455–456 (1988). https://doi.org/10.1007/BF02324793
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DOI: https://doi.org/10.1007/BF02324793