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Determination of bile acids in serum and bile by a modified gas chromatographic glass capillary technique

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Summary

A modified gas chromatographic glass capillary technique for determination of five major bile acids (Cholic acid: CA, Chenodeoxycholic acid: CDCA, Deoxycholic acid: DCA, Lithocholic acid: LCA and Ursodeoxycholic acid: UDCA) has been developed after preliminary extraction with XAD-2 resin. Enzymatic hydrolysis prevents the formation of interferring degradation products. Ether extraction with centrifugation eliminates water soluble interferring substances. Derivatization to hexafluorpropyl- instead of methyl esters results in better separation, shortens analysis time and prolongs the life span of the column.

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Authors and Affiliations

  1. Department of Internal Medicine II, Gastroenterology and Nutrition, University Clinic Ulm, D-7900, Ulm, FRG

    W. Swobodnik, W. Nebelung, U. Klueppelberg, J. G. Wechsler, P. Janowitz, H. Wenk & H. Ditschuneit

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  1. W. Swobodnik
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  2. W. Nebelung
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  3. U. Klueppelberg
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  4. J. G. Wechsler
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  5. P. Janowitz
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  6. H. Wenk
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  7. H. Ditschuneit
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Swobodnik, W., Nebelung, W., Klueppelberg, U. et al. Determination of bile acids in serum and bile by a modified gas chromatographic glass capillary technique. Chromatographia 25, 447–450 (1988). https://doi.org/10.1007/BF02324790

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  • DOI: https://doi.org/10.1007/BF02324790

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