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Chromatographia

, Volume 25, Issue 5, pp 447–450 | Cite as

Determination of bile acids in serum and bile by a modified gas chromatographic glass capillary technique

  • W. Swobodnik
  • W. Nebelung
  • U. Klueppelberg
  • J. G. Wechsler
  • P. Janowitz
  • H. Wenk
  • H. Ditschuneit
Originals

Summary

A modified gas chromatographic glass capillary technique for determination of five major bile acids (Cholic acid: CA, Chenodeoxycholic acid: CDCA, Deoxycholic acid: DCA, Lithocholic acid: LCA and Ursodeoxycholic acid: UDCA) has been developed after preliminary extraction with XAD-2 resin. Enzymatic hydrolysis prevents the formation of interferring degradation products. Ether extraction with centrifugation eliminates water soluble interferring substances. Derivatization to hexafluorpropyl- instead of methyl esters results in better separation, shortens analysis time and prolongs the life span of the column.

Key Words

Gas chromatography Glass capillary columns Bile acids Hexafluoroisopropyl esters 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1988

Authors and Affiliations

  • W. Swobodnik
    • 1
  • W. Nebelung
    • 1
  • U. Klueppelberg
    • 1
  • J. G. Wechsler
    • 1
  • P. Janowitz
    • 1
  • H. Wenk
    • 1
  • H. Ditschuneit
    • 1
  1. 1.Department of Internal Medicine II, Gastroenterology and NutritionUniversity Clinic UlmUlmFRG

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