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Process scale high-performance liquid chromatography Part II: Dissolution of silica matrices

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Summary

Possible contamination of purified product by the silica matrix is of considerable importance when considering process scale high-performance liquid chromatography (HPLC) as a separation methodology.

The stability of silica supports in normal phase, reversed phase and affinity chromatography modes has been examined with organic solvents and water. Both inorganic and organic components were found, contributed by the support, and the chromatographic and distillation equipment. In all cases, based on assumed values of production, they were found to contribute less than 0.01% of impurities. In critical cases crystallisation or dialysis may be considered as a last stage of purification, with re-chromatography to maximise yields.

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References

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Authors and Affiliations

  1. Fairmead, Legh Rd., WA16 8LS, Knutsford, Cheshire, UK

    K. Jones

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  1. K. Jones
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Additional information

This work was conducted in the Chemical Technology Unit, University of Manchester Institute of Science and Technology, P. O. Box 88, Manchester M60 1QD, UK.

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Jones, K. Process scale high-performance liquid chromatography Part II: Dissolution of silica matrices. Chromatographia 25, 443–446 (1988). https://doi.org/10.1007/BF02324789

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  • DOI: https://doi.org/10.1007/BF02324789

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