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Chromatographic behaviour of flavonoid compounds in reversed-phase HPLC systems

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Summary

Reversed-phase HPLC retention behaviour of different flavonoid compounds in different columns and with a range of methanol-buffer eluents was examined. Repeatibility and reproducibility of logk′ and Δlogk′ values were analyzed. Sharp group contributions, expressed as Δlogk′, were observed. These did not depend significantly on acid modifier type, column packing characteristics (C8 and C18) and dimensions (5,7 and 10μm) or the percentage of methanol in the mobile phase. A list of twelve group contributions is presented which is in agreement with literature data.

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Authors and Affiliations

  1. Analytical Chemistry Laboratory, Department of Chemistry, University of Ferrara, Via L. Borsari 46, I-44100, Ferrara, Italy

    F. Dondi, G. Blo, G. Lodi, C. Pietrogrande & P. Reschiglian

  2. Analytical Chemistry, Faculty of Industrial Chemistry, Somali National University, P.O. Box 1081, Mogadishu, Somalia

    Y. D. Kahie

Authors
  1. F. Dondi
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  2. G. Blo
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  3. Y. D. Kahie
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  4. G. Lodi
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  5. C. Pietrogrande
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  6. P. Reschiglian
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Dondi, F., Blo, G., Kahie, Y.D. et al. Chromatographic behaviour of flavonoid compounds in reversed-phase HPLC systems. Chromatographia 25, 423–429 (1988). https://doi.org/10.1007/BF02324786

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  • DOI: https://doi.org/10.1007/BF02324786

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