Summary
A general chromatographic model has been set up starting from a set of equations based on the concept of the velocity of a solute along the column. The composition of the mobile phase is taken into account solely as a numerical factor entering into suitable equations and totally independent of the chemical-properties of the constituents. A few isocratic experimental runs are necessary as input data, and subsequently a small amount of computational effort is sufficient to make predictions of retention times under gradient elution conditions for solutes of whatever chemical structure. The prediction errors are dependent on the steepness of the linear gradient chosen but are, in any case, acceptably low.
Similar content being viewed by others
References
Cs. Horvath, W. Melander, I. Molnar, J. Chromatogr.125, 129 (1976).
Cs. Horvath, W. Melander, I. Molnar, Anal. Chem.49, 142 (1977).
D. E. Martire, R. E. Boehm, J. Liq. Chromatogr.3, 753 (1980).
R. E. Koopmans, R. F. Rekker, J. Chromatogr.285, 267 (1984).
R. M. Smith, J. Chromatogr.209, 1 (1981).
W. E. Hammers, G. J. Meurs, C. L. De Ligny, J. Chromatogr.247, 1 (1982).
R. M. Carlson, R. E. Carlson, H. L. Koppermann, J. Chromatogr.107, 219 (1975).
K. Miyake, H. Terada, J. Chromatogr.240, 9 (1982).
K. Jinno, Chromatographia17, 367 (1983).
J. L. Thus, J. C. Kraak, J. Chromatogr.320, 271 (1985).
R. B. Sleight, J. Chromatogr.83, 31 (1973).
A. Bondi, J. Phys. Chem.68, 441 (1964).
K. Jinno, K. Kawasaki J. Chromatogr.316, 1 (1984).
K. Jinno, K. Kawasaki, J. Chromatogr.298, 326 (1984).
K. Jinno, K. Kawasaki, Chromatographia17, 337, 445 (1983).
K. Jinno, K. Kawasaki, Chromatographia17, 90, 211 (1984).
R. M. Smith, J. Chromatogr.236, 313 (1982).
J. K. Baker, Anal. Chem.51, 1693 (1979).
W. L. Stahovec, K. Mopper, J. Chromatogr.298, 399 (1984).
M. C. Gennaro, E. Mentasti, C. Sarzanini, V. Porta, Chromatographia,25, 117 (1988).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Marengo, E., Gennaro, M.C., Baiocchi, C. et al. A new approach to the prediction of RP-HPLC retention times in gradient elution from isocratic data. Chromatographia 25, 413–418 (1988). https://doi.org/10.1007/BF02324784
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02324784