, Volume 25, Issue 5, pp 409–412 | Cite as

Preparation of active ribosomal proteins by HPLC

  • Petra Nowotny
  • Helga Eckardt
  • V. Nowotny
  • Roza Maria Kamp


The proteins of the large ribosomal subunit fromEscherichia coli have been separated by size-exclusion, ion-exchange and reversed-phase high-performance-liquid chromatography (HPLC) using various buffer systems. The biological activity of the isolated proteins was tested via their ability to assemble into active 50S subunits (total reconstitution). The activity of the reconstituted subunits was measured with poly(U)-dependent poly-(Phe) synthesis. Reversed-phase HPLC techniques yielded active proteins (80–100%) by application of 2-propanol or acetonitrile. Proteins prepared by size-exclusion chromatography employing ammonium acetate as buffer also gave highly active proteins (70%). On the other hand, separation of the proteins on ion-exchange columns, using urea containing buffers, resulted in reduced activity (up to 50%).

Key Words

Column liquid chromatography Separation of proteins Biological activity after separation 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1988

Authors and Affiliations

  • Petra Nowotny
    • 1
  • Helga Eckardt
    • 1
  • V. Nowotny
    • 1
  • Roza Maria Kamp
    • 1
  1. 1.Abt. WittmannMax-Planck-Institut für Molekulare GenetikBerlin 33FRG

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