, Volume 25, Issue 4, pp 339–342 | Cite as

Optimization of stepwise gradient elution in columns chromatography

  • W. Markowski
  • W. Gołkiewicz
Last Posters from the Amsterdam Symposium HPLC 87


A general equation for the final retention of a solute chromatographed under conditions of stepwise gradient elution has been derived. The elution process and the distances travelled by solutes as a function of eluent volume were simulated by computer for the optimization of stepwise gradient prorams from isocratic HPLC data. The validity of the equations was experimentally veritied.

Key Words

Column liquid chromatography Gradient elution Prediction of retention 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1988

Authors and Affiliations

  • W. Markowski
    • 1
  • W. Gołkiewicz
    • 1
  1. 1.Department of Inorganic and Analytical ChemistryMedical AcademyLublinPoland

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