Summary
A general equation for the final retention of a solute chromatographed under conditions of stepwise gradient elution has been derived. The elution process and the distances travelled by solutes as a function of eluent volume were simulated by computer for the optimization of stepwise gradient prorams from isocratic HPLC data. The validity of the equations was experimentally veritied.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
L. R. Snyder, in “High Performance Liquid Chromatography”, Adv. and Persp., C. Horvath-Editor, Academic Press, Inc., pp. 207–316 (1980).
P. Jandera, J. Churacek, in “Gradient Elution in Column Liquid Chromatography”, Elsevier, (1985).
W. Gołkiewicz, M. Jaroniec, J. High Resolut. Chromatogr. and Chromatogr. Commun.,4, 245 (1978).
W. Golkiewicz, Chromatographia14, 411, 1981;14, 629 (1978).
E. Soczewiński, W. Markowski, J. Chromatogr.,370, 63 (1986).
W. Markowski, E. Soczewiński, G. Matysik, J. Liq. Chromatogr., in press.
L. R. Snyder, J. J. Kirkland, in “Introduction to Modern Liquid Chromatography”, Second Edition, J. Wiley and Sons, Inc., New York 1979.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Markowski, W., Gołkiewicz, W. Optimization of stepwise gradient elution in columns chromatography. Chromatographia 25, 339–342 (1988). https://doi.org/10.1007/BF02324712
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02324712