, Volume 27, Issue 7–8, pp 371–377 | Cite as

Selectivity of alkyl-bonded phases in reversed-phase liquid chromatography of hydrophobic compounds

  • J. Yamaguchi
  • T. Hanai
Late Papers from the 17th International Symposium on Chromatography, Vienna, Austria, September 25–30, 1988


Starting from silica gel with a purity of 99.999%, octadecyl-(ODS), octyl-(C8) and propyl-(C3) bonded silica gels were synthesized, and end-capped by trimethyl groups. The pore size was 150 Å and the specific surface area was 320 m2/g. The ODS and C8 silica gels were stable in both acidic and basic solutions.

The selectivity of these bonded-silica gels was examined from the difference of the retention behavior of alkanes (Al−Al), alkylbenzenes (Ph−Al), alkylalcohols (Al−OH) and polyaromatic hydrocarbons (PAH) in aqueous acetonitrile. On these packings the log k′ values decreased in the order Al−Al>Ph−Al>PAH>Al−OH. Furthermore, the chromatographic behavior of these compounds was measured on polymer-coated octadecyl-bonded silica gel (CAP) and octadecyl-bonded vinyl alcohol copolymer gel (ODP).

The selectivity was verified from the value of R* of these compounds:
$$R = R_v + R^* $$
where R is the retention time, and Rv is the retention time of an alkane having the same Van der Waals volume as the analyte. The R* values indicated that this ODS had more selectivity than C8 and C3, however R* on C8 showed a very good correlation with R* on C3.

Their retention behavior on CAP was similar to that of ODS, however, their capacity ratios measured on CAP were smaller than those on ODS. The ODP has selectivity for the retention of aromatic compounds, because PAHs were retained strongly on this column.

Key words

Column liquid chromatography Alkyl-bonded silica gel Enthalpy Van der Waals volume 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1989

Authors and Affiliations

  • J. Yamaguchi
    • 1
  • T. Hanai
    • 1
  1. 1.Gasukuro Kogyo Inc.IrumaJapan

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