Summary
The advantages of an evaporative light scattering detector in HPLC are demonstrated using the separation of α, ω-dicarboxylic and hydroxycarboxylic acids, alkyl glucosides and mono-, di- and triglycerides as examples. By using this detector reversed phase as well as normal phase gradient HPLC becomes possible for substances with no UV absorption.
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Bruns, A., Waldhoff, H. & Winkle, W. Applications of HPLC with evaporative light scattering detection in fat and carbohydrate chemistry. Chromatographia 27, 340–342 (1989). https://doi.org/10.1007/BF02321281
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DOI: https://doi.org/10.1007/BF02321281