Summary
A gas chromatographic assay for spectinomycin hydrochloride is described. The method is based on that prescribed by the United States Pharmacopeia (USP XXII). The method involves silylation of spectinomycin hydrochloride; phenazone is used as an internal standard. Spectinomycin and phenazone have adequate stability under the prescribed conditions. The stationary phase is 3% OV-17 on Gaschrom Q 100–120 mesh. The selectivity of the proposed method is better than that of the GLC method described in the USP XXII.
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Hoebus, J., Yun, L.M. & Hoogmartens, J. An improved gas chromatographic assay for spectinomycin hydrochloride. Chromatographia 39, 71–73 (1994). https://doi.org/10.1007/BF02320461
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DOI: https://doi.org/10.1007/BF02320461