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Extraction, purification procedures and HPLC-RI analysis of carbohydrates in olive (Olea europaea L.) plants

Summary

Qualitative and quantitative analyses of carbohydrates in olive (Olea europaea L.) tissues have been carried out by HPLC-RI. Sample purification was by two successive solid-liquid extractions to remove completely plant phenolics and pigments. Five carbohydrate peaks; sucrose (stachyose + raffinose + sucrose), glucose, galactose, fructose (fructose + myo-inositol) and mannitol were detected when plant extracts were run on a Sugar SC 1011 column operating at 75°C, using water as eluent 0.5 ml min−1. The use of two serial Sugar SC 1011 columns operating at 90°C and eluting the plant extracts with H2O−CH3CN (95/5 v/v) enables identification and quantification of nine carbohydrates, including tetra and tri-saccharides.

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Romani, A., Baldi, A., Tattini, M. et al. Extraction, purification procedures and HPLC-RI analysis of carbohydrates in olive (Olea europaea L.) plants. Chromatographia 39, 35–39 (1994). https://doi.org/10.1007/BF02320455

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Key Words

  • Liquid chromatography
  • Olive tissues
  • Oligosaccharide quantitation
  • Refractive index detection