, Volume 30, Issue 5–6, pp 245–248 | Cite as

Trans-esterification of fatty acids from microorganisms and human blood serum by trimethylsulfonium hydroxide (TMSH) for GC analysis

  • K.-D. Müller
  • H. Husmann
  • H. P. Nalik
  • G. Schomburg


Trimethylsulfonium hydroxide (TMSH) can convert fatty acids into the corresponding fatty acid methyl esters (FAMEs) in a single step. These fatty acids may also be bound in biomolecules such as phospholipids and/or glycerides. Complex mixtures of saturated and unsaturated FAMEs which may contain hydroxy and cylopropyl groups are obtained by trans-esterification; they can easily be separated in most cases by capillary GC. When FAMEs are generated from different microorganisms e.g. bacteria the patterns of the chromatograms are characteristic. Examples of characteristic patterns of bacteria with different cell wall structures are shown. The described method of transesterification can also be applied directly to blood serum without sophisticated sample pretreatment. The profiles of the chromatograms match well those described in the literature obtained by other methods of trans-esterification or sample preparation.

Key Words

Gas chromatography Trimethylsulfonium hydroxide (TMSH) FAME analysis Bacteria Blood serum 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. [1]
    F. van Nieuwenhuyze, P. Sandra, in: Proceedings of the 8th International Symposium on Capillary Chromatography, Riva del Garda 1987,P. Sandra (Ed.), Dr. A. Huethig Verlag, Heidelberg 1987, p. 764.Google Scholar
  2. [2]
    L. Miller, T. Berger, Hewlett-Packard Application Note 228-41 (1985) 8 pp.Google Scholar
  3. [3]
    W. Butte, J. Chromatogr.,261, 142 (1983).CrossRefGoogle Scholar
  4. [4]
    E. Schulte, K. Weber, Fat. Sci. Technol.,91, 181 (1989).Google Scholar
  5. [5]
    L. Matter, D. Schenker, H. Husmann, G. Schomburg, Chromatographia27, 31 (1989).CrossRefGoogle Scholar
  6. [6]
    K.-D. Müller, H. Husmann, H. P. Nalik, Zbl. Bakt., in press.Google Scholar
  7. [7]
    C. W. Moss, J. Chromatogr.203, 337 (1981).Google Scholar
  8. [8]
    M. A. Lambert, C. W. Moss, J. Clin. Microbiol.,18, 1370 (1983).Google Scholar
  9. [9]
    H. Jaeger, W. Wagner, J. Homoki, H. U. Klör, H. Ditschuneit, in: Glass capillary chromatography, 2nd Internatinal Symposium, Hindelang, 1977,R. E. Kaiser (Ed.), Institute of Chromatography, Bad Dürkheim, 1977, p. 129.Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1990

Authors and Affiliations

  • K.-D. Müller
    • 1
  • H. Husmann
    • 2
  • H. P. Nalik
    • 1
  • G. Schomburg
    • 2
  1. 1.Institut für Medizinische MikrobiologieUniversität-Gesamthochschule (GHS) EssenEssenFRG
  2. 2.Abteilung ChromatographieMax-Planck-Institut für KohlenforschungMülheim an der RuhrFRG

Personalised recommendations