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Simultaneous flow rate and temperature programming based on computer-assisted retention prediction for the separation of PTH-amino acids in reversed-phase microcolumn liquid chromatography

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Summary

The separation of phenylthiohydantoin (PTH) amino acid derivatives by flow rate and temperature programming in reversed-phase micro-column liquid chromatography (LC) has been investigated. For both programming modes a microcomputer-assisted retention prediction system (REPRES) was constructed. The performance of this system has been evaluated by comparing actually measured retention data to those predicted. Excellent agreements between those data were found. It is clearly indicated that simultaneous flow rate and temperature programming can improve the separation of PTH-amino acids in reduced analysis time.

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Authors and Affiliations

  1. School Materials Science, Toyohashi University of Technology, 440, Toyohashi, Japan

    K. Jinno & M. Yamagami

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  1. K. Jinno
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  2. M. Yamagami
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Jinno, K., Yamagami, M. Simultaneous flow rate and temperature programming based on computer-assisted retention prediction for the separation of PTH-amino acids in reversed-phase microcolumn liquid chromatography. Chromatographia 27, 417–424 (1989). https://doi.org/10.1007/BF02319557

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  • DOI: https://doi.org/10.1007/BF02319557

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