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Verfahren zur Herstellung und verlustlosen Dosierung von Derivaten für die Gas-Chromatographie. Ein Beitrag zur gas-chromatographischen Aminosäure-Analyse

Procedure for the preparation and dosage, without loss, of derivatives for gas chromatography. A contribution to gas chromatographic analysis of amino acids

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Summary

A procedure is described for sample handling in gas chromatography, where a capsule of aluminium or gold is used as a micro-reactor for chemical reactions necessary for the preparation of volatile derivatives. This capsule is used at the same time for sample transfer into the gas chromatograph. This enables the user to carry out derivatization of small sample amounts without any loss of substance, and to transfer the total sample volume into the gas chromatograph for further analysis. As an example of the many possibilities the use of this procedure for the preparation of amino acid derivatives is described.

Zusammenfassung

Es wird ein Verfahren für die Probenhandhabung in der Gas-Chromatographie beschriben, bei dem eine Kapsel aus Aluminium oder Gold as Mikroeaktor für chemische Umsetzungen zur Herstellung von flüchtigen Derivaten verwendet wird. Diese Kapsel dient gleichzeitig zur Probenüberführung in den Gas-Chromatographen. Damit lassen sich geringe Substanzmengen verlustlos derivatisieren und vollständig in den Gas-Chromatographen zur weiteren Analyse überführen. Als Beispiel für die vielfältigen Möglichkeiten wird die Verwendung dieses Verfahrens zur Herstellung von Aminosäurederivaten angegeben.

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Authors and Affiliations

  1. Bodenseewerk Perkin-Elmer & Co GmbH, Überlingen, Germany

    B. Kolb & W. Hoser

Authors
  1. B. Kolb
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  2. W. Hoser
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Kolb, B., Hoser, W. Verfahren zur Herstellung und verlustlosen Dosierung von Derivaten für die Gas-Chromatographie. Ein Beitrag zur gas-chromatographischen Aminosäure-Analyse. Chromatographia 6, 28–31 (1973). https://doi.org/10.1007/BF02319388

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  • DOI: https://doi.org/10.1007/BF02319388

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