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Plant Cell, Tissue and Organ Culture

, Volume 47, Issue 3, pp 269–278 | Cite as

Sucrose uptake and metabolism in a double layer system for micropropagation ofRosa multiflora

  • Jan De Riek
  • Abel Piqueras
  • Pierre C. Debergh
Original Research Papers

Abstract

Uptake and metabolism of sucrose in micropropagatedRosa multiflora using the double layer technique was investigated. In the multiplication as well as the root induction stage, hydrolysis of sucrose in the culture medium was observed. A mathematical model was developed to quantify sucrose hydrolysis and the uptake of sucrose, glucose and fructose, based on the time series for the different sugars in the culture medium. These data were linked to a study of the sugar metabolism in the microshoots. After 48 h of incubation on14C-[U]-glucose containing medium, the incorporated label was mainly detected in the ethanol soluble fraction; within this fraction sucrose was the most important compound. This indicates a significant re-synthesis of sucrose in the plant material after the uptake of hexose. To assess the extent that different enzymes of sucrose metabolism (invertases, sucrose synthase and sucrose-P-synthase) were involved, their activity in different plant parts (of final stage III microshoots) were assayed. A decreasing gradient for sucrose metabolising enzymes from the roots toward the leaves gave a good indication of how the different tissues depend on sucrose absorbed from the medium.

Key words

invertase micropropagation Rosa multiflora sucrose metabolism sucrose synthase sucrose-P-synthase 

Abbreviations

AI

acid invertase

CW

cell wall fraction

NI

neutral invertase

SF

soluble fraction

SPS

sucrose phosphate synthase

SS

sucrose synthase

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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Jan De Riek
    • 1
  • Abel Piqueras
    • 1
  • Pierre C. Debergh
    • 1
  1. 1.Department of Plant Production - HorticultureUniversity of GentGentBelgium

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