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Plantlets from encapsulated micropropagated buds of M.26 apple rootstock

Abstract

In order to be considered usable as synthetic seeds, encapsulated explants sown underin vitro orex vitro conditions must be able to produce whole plantlets. Ninety percent of non-encapsulated M.26 apple rootstock single nodes produced a plantlet (i.e., a well-formed shoot with a root system) after 30 days of culturein vitro if the explants were previously given a 24-hour treatment with 24.6 µM IBA and 15 g 1−1 sucrose in darkness. In contrast, when the single nodes were encapsulated in a calcium-sodium alginate bead immediately after the same treatment only 10% of the encapsulated explants formed a plantlet. Addition of growth regulators to the artificial endosperm and culture of the single nodes for root primordia initiation for 3, 6 or 9 days in darkness before encapsulation allowed production of 58%, 60% and 66% of plantlets, respectively.

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Abbreviations

AdSO4 :

adenine hemisulfate

BA:

benzyladenine

GA3 :

gibberellic acid

IBA:

indolebutyric acid

PVP:

polyvinyl pyrrolidone

Synseed:

synthetic seed

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Piccioni, E. Plantlets from encapsulated micropropagated buds of M.26 apple rootstock. Plant Cell Tiss Organ Cult 47, 255–260 (1997). https://doi.org/10.1007/BF02318980

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  • DOI: https://doi.org/10.1007/BF02318980

Key words

  • artificial endosperm
  • Malus
  • regrowth
  • rooting
  • synthetic seed