Plant Cell, Tissue and Organ Culture

, Volume 47, Issue 3, pp 231–238 | Cite as

Clonal propagation byin vitro culture of Corchorus (jute)

  • Ali M. Abbas
  • J. K. Jones
  • P. D. S. Caligari
Original Research Papers

Abstract

Callus was produced on cotyledon, shoot tip, hypocotyl and root explants of twoCorchorus species on several media. Cytokinin was necessary for callus production on cotyledon explants. BothC.olitorius genotypes produced most callus on media with zeatin and either NAA or IAA, and theC.capsularis genotype produced most callus on media with IAA and either zeatin or BA. High frequencies of regenerated shoots were obtained from shoot tip explants of both species, from the apical meristem and from callus. Media with 2.0 mg 1−1 BA were superior for both species, and media with zeatin were equally good forC.capsularis only. More regeneration was obtained for all genotypes after subculture of callus on media with 2.0 mg 1−1 zeatin. Cotyledon callus produced less regeneration, also with differences between genotypes; explants of both genotypes ofC.olitorius produced regeneration on a medium with NAA and zeatin, and theC.capsularis genotype produced regeneration on a medium with IAA and BA. Limited regeneration from root explant callus was obtained forC.capsularis only on medium with BA and IAA. Regeneration was not obtained from hypocotyl callus. Further regeneration of shoots of both species was obtained from secondary callus after subculture, and from nodal segments of regenerated shoots and of seedling shoots cultured on basic MS medium without growth hormones. Roots were produced on about 80% of all shoots after transference to medium with 0.2 mg 1−1 IBA, and rooted plantlets survived and flowered normally after transference to compost.

Key words

Clonal propagation Corchorus tissue culture regeneration nodal explants multiplication jute 

Abbreviations

MS

Murashige and Skoog (1962)

BA

6-benzylaminopurine

IAA

indole-3-acetic acid

IBA

indole-3-butyric acid

NAA

naphthalene acetic acid

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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Ali M. Abbas
    • 2
  • J. K. Jones
    • 1
  • P. D. S. Caligari
    • 1
  1. 1.Department of Agricultural Botany, School of Plant SciencesThe University of ReadingReadingUK
  2. 2.Breeding DivisionBangladesh Jute Research InstituteDhakaBangladesh

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