Clonal propagation byin vitro culture of Corchorus (jute)
- 198 Downloads
Callus was produced on cotyledon, shoot tip, hypocotyl and root explants of twoCorchorus species on several media. Cytokinin was necessary for callus production on cotyledon explants. BothC.olitorius genotypes produced most callus on media with zeatin and either NAA or IAA, and theC.capsularis genotype produced most callus on media with IAA and either zeatin or BA. High frequencies of regenerated shoots were obtained from shoot tip explants of both species, from the apical meristem and from callus. Media with 2.0 mg 1−1 BA were superior for both species, and media with zeatin were equally good forC.capsularis only. More regeneration was obtained for all genotypes after subculture of callus on media with 2.0 mg 1−1 zeatin. Cotyledon callus produced less regeneration, also with differences between genotypes; explants of both genotypes ofC.olitorius produced regeneration on a medium with NAA and zeatin, and theC.capsularis genotype produced regeneration on a medium with IAA and BA. Limited regeneration from root explant callus was obtained forC.capsularis only on medium with BA and IAA. Regeneration was not obtained from hypocotyl callus. Further regeneration of shoots of both species was obtained from secondary callus after subculture, and from nodal segments of regenerated shoots and of seedling shoots cultured on basic MS medium without growth hormones. Roots were produced on about 80% of all shoots after transference to medium with 0.2 mg 1−1 IBA, and rooted plantlets survived and flowered normally after transference to compost.
Key wordsClonal propagation Corchorus tissue culture regeneration nodal explants multiplication jute
Murashige and Skoog (1962)
naphthalene acetic acid
Unable to display preview. Download preview PDF.
- Gawal NJ & Robacker CD (1990) Somatic embryogenesis in twoGossypium hirsutum genotypes on semi solid versus liquid media. Plant Cell Tiss. Org. Cult. 23: 201–204Google Scholar
- Islam AS (1981) Production of desirable jute plants through tissue culture. Proc. COSTED Symp. on Tissue Culture of Economically Important Plants. Singapore. Ed. A N Rao. 159–161Google Scholar
- Islam AS, Haque MM, Haque MI & Seraj ZI (1992) Tissue culture and micropropagation of jute (Corchorus spp). In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry, High-Tech and Micropropagation III Vol.19, (pp.505–526) Springer Verlag Berlin, HeidelbergGoogle Scholar
- Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plantarum 15: 473–497Google Scholar
- Rahman ZSM, Hadiuzzaman S, Haque MM & Islam AS (1985) Shoot formation inCorchorus capsularis var. D-154 from unorganised cells. Bangla. J.Bot. 14: 141–145Google Scholar
- Saba T & Sen SK (1992) Somatic embryogenesis in protoplast derived calluses of cultivated jute,Corchorus capsularis L. Plant Cell Rep. 10: 633–636Google Scholar