Plant Cell, Tissue and Organ Culture

, Volume 47, Issue 2, pp 153–158 | Cite as

Regeneration of haploid plants from anther cultures of the Asiatic hybrid lily ‘Connecticut King’

  • Dong-Sheng Han
  • Yoshiji Niimi
  • Masalu Nakano
Original Research Papers


A system for producing haploid plants from anther cultures was developed for the Asiatic hybrid lily ‘Connecticut King’. Anthers containing microspores at the mid- to late-uninucleate stages were cultured on MS media supplemented with various plant growth regulators. Microspores containing 3 or 4 vegetative-like nuclei were observed 2 to 3 weeks later, and yellowish nodular calluses appeared within dehisced anthers 2 to 3 months after culture. Picloram was superior to 2,4-d for inducing nodular calluses. Anthers from greenhouse-grown plants required higher concentrations of both picloram and cytokinins than those from field-grown plants and most frequently produced nodular calluses (17.6%) on MS medium containing 2 mg 1−1 picloram and 2 mg 1−1 zeatin. The nodular calluses regenerated many bulblets following transfer to MS medium supplemented with 0.1 or 0.5 mg 1−1 picloram and 0.01 mg 1−1 BA, and the bulblets developed into plantlets (bulblets with scaly leaves and roots) after transfer to MS medium containing 0.1 mg 1−1 NAA. Chromosome counts of root-tip cells of 11 plantlets revealed that five were haploids (2n = 12), two diploids (2n = 24), and four mixoploid. This result suggests that at least some plantlets were of gametophytic origin.

Key words

androgenesis Liliaceae picloram 





2,4-dichlorophenoxyacetic acid




Murashige and Skoog (1962)


α-naphthaleneacetic acid


4-amino-3,5,6-trichloropicolinic acid


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  1. Beyl CA & Sharma GC (1983) Picloram induced somatic embryogenesis inGasteria andHaworthia. Plant Cell Tiss. Org. Cult. 2: 123–132CrossRefGoogle Scholar
  2. Feng XR & Wolyn DJ (1991) High frequency production of haploid embryos in asparagus anther culture. Plant Cell Rep. 10: 574–578CrossRefGoogle Scholar
  3. Gu Z-P & Cheng K-C (1982) Studies on induction of pollen plantlets from the anther cultures of lily. Acta Bot. Sin. 24: 28–32Google Scholar
  4. Jähne A & Lörz H (1995) Cereal microspore culture. Plant Sci. 109: 1–12Google Scholar
  5. Keller WA, Arnison PG & Cardy BJ (1987) Haploid from gametophytic cells - recent developments and future prospects. In: Green CE, Somers DA, Hackett WP and Biesboer DD (Eds) Plant Tissue and Cell Culture (pp 223–241). A R Liss New YorkGoogle Scholar
  6. Maheshwari SC, Tyagi AK, Malhotra K & Sopory SK (1980) Induction of haploidy form pollen grains in angiosperms - the current status. Theor. Appl. Genet. 58: 193–206CrossRefGoogle Scholar
  7. Maheshwari SC, Rashid A & Tyagi AK (1987) Haploids from pollen grains - retrospect and prospect. Amer. J. But. 69: 865–879Google Scholar
  8. Morrison RA & Evans DA (1988) Haploid plants from tissue culture: new plant varieties in a shortened time frame. Bio/Technology 6: 684–690CrossRefGoogle Scholar
  9. Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–479Google Scholar
  10. Phillips GC & Luteyn KJ (1983) Effects of picloram and other auxins on onion tissue cultures. J. Amer. Soc. Hort. Sci. 108: 948–953Google Scholar
  11. Prakash J & Giles KL (1986) Production of doubled haploids in oriental lilies. In: Horn W, Jensen CJ, Odenbach W and Schieder O (Eds), Genetic Manipulation in Plant Breeding (pp 335–337). de Gruyter, BerlinGoogle Scholar
  12. Qu Y, Mok MC, Mok DWS & Stang JR (1988) Phenotypic and cytological variation among plants derived from anther cultures ofLilium longifiorum. In Vitro Cell. Dev. Biol. 24: 471–476Google Scholar
  13. Sharp WR, Raskin RS & Sommer HE (1971) Haploidy inLilium. Phytomorphology 21: 334–335Google Scholar
  14. Van Tuyl JM (1992) Survey research on mitotic and meiotic polyploidization at CPRO-DLO. The Lily Yearbook of the North Amer. Lily Soc. 45: 10–18Google Scholar

Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Dong-Sheng Han
    • 1
  • Yoshiji Niimi
    • 1
  • Masalu Nakano
    • 1
  1. 1.Faculty of AgricultureNiigata UniversityIkarashi, NiigataJapan

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