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Plant Cell, Tissue and Organ Culture

, Volume 1, Issue 1, pp 211–219 | Cite as

Isolation of protoplasts from somatic embryos of carrot

  • K. Nomura
  • T. Nitta
  • T. Fujimura
  • A. Komamine
Article

Abstract

Protoplasts were isolated enzymatically from synchronously induced globular somatic embryos from a carrot suspension culture. Among the macerating enzymes tested, Driselase was the most effective for release of protoplasts from embryos. A higher medium osmolarity was required for the isolation of protoplasts from embryos than from undifferentiated cells. Protoplasts from embryos were smaller than protoplasts from undifferentiated cells. On step gradients of Ficoll, protoplasts from embryos gave one major band. Protoplasts from undifferentiated cells gave two major bands, one lighter and the other heavier than the protoplasts from embryos.

Key words

carrot somatic embryo protoplast cell density 

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References

  1. 1.
    Fujimura T, Komamine A (1975) Effects of various growth regulators on the embryogenesis in a carrot cell suspension culture. Plant Sci Lett 5:359–364Google Scholar
  2. 2.
    Fujimura T, Komamine A (1979) Synchronization of somatic embryogenesis in a carrot cell suspension culture. Plant Physiol 64:162–164Google Scholar
  3. 3.
    Lin M, Staba J (1961) Peppermint and spearmint tissue cultures. I. Callus formation and submerged culture. Lloydia 24:139–145Google Scholar
  4. 4.
    Vasil V, Vasil IK (1980) Isolation and culture of cereal protoplasts. Theor Appl Genet 56:97–99CrossRefGoogle Scholar

Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1981

Authors and Affiliations

  • K. Nomura
    • 1
  • T. Nitta
    • 1
  • T. Fujimura
    • 2
  • A. Komamine
    • 2
  1. 1.Laboratory of Biology, Faculty of General EducationTokyo University of Agriculture and TechnologyFuchu, TokyoJapan
  2. 2.Department of Botany, Faculty of ScienceUniversity of TokyoTokyoJapan

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