Neue mehrfache Untersuchungen am gleichen Kammerwasser menschlicher Augen

Summary

The normal human aequous has a volume of 0.12–0.20 cm³ and its protein content is from 10 to 20 mg%. We ascertained the specific weight by the falling drop method in a mixture of xylol and brombenzene. In order to estimate the protein content, we placed a series of drops from the human aequous on filter paper with the Agla pipette; they were then tinted with amido-black and the optical density of the eluted spots was read in the Beckman spectrophotometer. The remnants of 2–3 specimens of human aequous were then brought together and concentrated there until the protein concentration is just sufficient to allow a separation into the various fractions by paper-electrophoresis.