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Selection of optimal pH and solvent composition for the separation of organic acids using three-dimensional diagrams and a computer program

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The retention behaviour of different types of organic acids was investigated on an ODS-Hypersil column using eluents of various pH and of various methanol-water ratios. Equations introduced by Horvath and coworkers were fitted onto the experimental data, and the obtained constants were used to calculate the three-dimensional k′-surfaces for the compounds in the investigated pH and solvent composition range. Using the data of the individual surface diagrams the optimal pH and solventg composition was computed for the separation of six selected compounds. To verify the applicability of the above procedure the chromatographic separation of the six compounds was carried out with the predicted conditions, and the chromatogram obtained is presented.

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Authors and Affiliations

  1. Institute for Analytical Chemistry, University of Chemical Engineering, Post Office Box 158, H-8201, Veszprém, Hungary

    F. Szokoli, Zs. Németh & J. Inczédy

Authors
  1. F. Szokoli
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  2. Zs. Németh
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  3. J. Inczédy
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Szokoli, F., Németh, Z. & Inczédy, J. Selection of optimal pH and solvent composition for the separation of organic acids using three-dimensional diagrams and a computer program. Chromatographia 29, 265–268 (1990). https://doi.org/10.1007/BF02317916

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  • DOI: https://doi.org/10.1007/BF02317916

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