Summary
New approaches to the isolation and purification of plant and animal pesticide metabolites have been developed. Polar conjugates (glycosides, glucuronides, sulfate esters, etc.) from plant or animal metabolism studies have been purified either as the intact conjugates or as derivatives of the enzyme hydrolyzed conjugates by using a combination of high performance liquid chromatography or gas liquid chromatography. The methods developed for the isolation and characterization of the plant and animal isopropyl-3-chlorocarbanilate metabolites are described.
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Mention of a trade name, proprietary products, or specific equipment does not constitute a guarantee or warranty by the U. S. Department of Agriculture and does not apply its approval to the exclusion of other products that may be suitable.
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Still, G.G., Mansager, E.R. High performance liquid and gas liquid chromatography applied to the purification of pesticide plant and animal metabolites. Chromatographia 8, 129–135 (1975). https://doi.org/10.1007/BF02316562
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DOI: https://doi.org/10.1007/BF02316562