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The role of thruput in high-performance liquid chromatography

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Summary

A systematic study of high performance preparative liquid chromatography demonstrated that increased solute thruput (mg/min) is gained by use of high sample load (mg solute/g packing) and high carrier velocity. A plot of HETP vs normalized load indicated that peak width increases as a function of load but that the separation process is maintained. Increased loadability is gained by increased retention.

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Authors and Affiliations

  1. Tracor, Inc., 78721, Austin, Texas, U.S.A.

    K. J. Bombaugh & P. W. Almquist

Authors
  1. K. J. Bombaugh
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  2. P. W. Almquist
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Bombaugh, K.J., Almquist, P.W. The role of thruput in high-performance liquid chromatography. Chromatographia 8, 109–118 (1975). https://doi.org/10.1007/BF02316558

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  • DOI: https://doi.org/10.1007/BF02316558

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