Summary
A rapid and efficient procedure is described for the extraction and analysis of codeine and morphine in whole blood. Red blood cells were fragmented by sonication and the blood sample extracted by passing through a bonded silica column (Bond Elute®). The adsorbed drugs were washed and eluted followed by analysis by HPLC. Recoveries were between 95–100% at 5 ng/ml concentrations.
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Tebbett, I.R. Rapid extraction of codeine and morphine in whole blood for HPLC analysis. Chromatographia 23, 377–378 (1987). https://doi.org/10.1007/BF02316187
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DOI: https://doi.org/10.1007/BF02316187