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Gel filtration: A method for desalting and group separation

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Abstract

We wish to report a simple and rapid method for the fractionation of water-soluble substances. The method is based on a column procedure similar to chromatography in which the stationary phase is comprised of a new type of gel. These gels consist of hydrophilic chains which are cross-linked. They are devoid of ionic groups, the polar character being almost entirely due to the high content of hydroxyl groups. While water-insoluble, the gels are never-theless capable of considerable swelling. The fractionation depends primarily on differences in molecular size although phenomena have been observed which indicate the influence of other factors.

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Authors and Affiliations

  1. Institute of Biochemistry, University of Uppsala, Uppsala, Sweden

    Jerker Porath

  2. Research Laboratories, Pharmacia, Uppsala

    Per Flodin

Authors
  1. Jerker Porath
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  2. Per Flodin
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Porath, J., Flodin, P. Gel filtration: A method for desalting and group separation. Chromatographia 23, 365–369 (1987). https://doi.org/10.1007/BF02316184

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  • DOI: https://doi.org/10.1007/BF02316184

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