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Optimization of fat soluble vitamin separation in reversed-phase microcolumn liquid chromatography

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Summary

Separation behavior of fat soluble vitamins on various chemically bonded materials in non-aqueous reversedphases microcolumn liquid chromatography has been investigated. The best performance was found on a polymer-based octadecyl bonded phase. Optimization of separation for vitamins D2, D3, E and E acetate has been tried using this polymer-based column and it has been found that a binary mobile phase consisting of acetonitrile and methanol gave complete separation of fat soluble vitamins.

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References

  1. W. O. Landen Jr., J. Chromatogr.,211, 155 (1981).

    Article  CAS  Google Scholar 

  2. R. vanHaelen-Fastré, M. vanHaelen, J. Chromatogr.,179, 131 (1979).

    Article  Google Scholar 

  3. W. J. Driskell, J. W. Neese, C. C. Bryant, M. M. Bashor, J. Chromatogr.,231, 439 (1982).

    CAS  Google Scholar 

  4. C. A. Collins, C. K. Chow, J. Chromatogr.,317, 349 (1984).

    Article  CAS  Google Scholar 

  5. P. J. van Niekerk, S. C. C. Smit, J. A. O. C. S.,57, 12, 417 (1980).

    Google Scholar 

  6. M. K. Head, E. Gibbs, J. Food Sci.,42, 2, 395 (1977).

    Google Scholar 

  7. S. A. Barnett, L. W. Frick, H. M. Baine, Anal. Chem.,52, 610 (1980).

    Article  CAS  Google Scholar 

  8. D. Thorburn Burns, C. Mackay, J. Tillman, J. Chromatogr.,190, 141 (1980).

    Google Scholar 

  9. F. Zonta, B. Stancher, J. Bielawny, J. Chromatogr.,246, 105 (1982).

    Article  CAS  Google Scholar 

  10. V. M. Novotny, D. Ishii (Eds.), “Microcolumn Separation, Column, Instrumentation and Ancillary Techniques”, J. Chromatogr. Library, No. 30, Elsevier, Amsterdam (1985).

    Google Scholar 

  11. P. Kucera (Ed.), “Microcolumn High-Performance Liquid Chromatography”, J. Chromatogr. Library, vol. 28. Elsevier Amsterdam (1983).

    Google Scholar 

  12. W. T. Wahyuni, K. Jinno, J. Micronutrient Anal.,3, (1), 41 (1987).

    Google Scholar 

  13. K. Jinno, T. Nagoshi, N. Tanaka, M. Okamoto, J. C. Fetzer, W. R. Biggs, J. Chromatogr.,386, 123 (1987).

    CAS  Google Scholar 

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Authors and Affiliations

  1. School of Materials Science, Toyohashi University of Technology, 440, Toyohashi, Japan

    W. T. Wahyuni & K. Jinno

Authors
  1. W. T. Wahyuni
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  2. K. Jinno
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Wahyuni, W.T., Jinno, K. Optimization of fat soluble vitamin separation in reversed-phase microcolumn liquid chromatography. Chromatographia 23, 320–324 (1987). https://doi.org/10.1007/BF02316175

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  • DOI: https://doi.org/10.1007/BF02316175

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