Summary
This work shows the influence of the injection volume on peak broadening owing to the increase of the sample concentration; use of the maximum injection volume is advocated. Once the chromatographic system is defined, a method for calculating the dimensions of the preparative column is developed. Applications of this method are shown.
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References
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Coq, B., Cretier, G., Gonnet, C. et al. How to approach preparative liquid chromatography. Chromatographia 12, 139–146 (1979). https://doi.org/10.1007/BF02314866
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DOI: https://doi.org/10.1007/BF02314866