A thin-layer chromatographic procedure for estimating traces of copper in edible fats
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To carry out estimations as stated in the title, the sample matrix was first destroyed by an improved incineration procedure. Very small quantities of copper (not exceeding, however, 0.03 μg/cm in the chromatogram) were estimated by measurement of fluorescence after spraying with an ammonium phosphate reagent. Larger quantities (up to 0.3 μg/cm) were estimated on the basis of copper-induced quenching of fluorescence produced by spraying the developed chromatograms with glucose. In these experiments the metal is located as non-fluorescent areas on a yellowish-green fluorescent background.
KeywordsGlucose Copper Phosphate Ammonium Analytical Chemistry
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