Inflammation Research

, Volume 45, Issue 12, pp 583–589 | Cite as

Effects of three different Ca2+-ATPase inhibitors on Ca2+ response and leukotriene release in RBL-2H3 cells

  • R. Akasaka
  • R. Teshima
  • H. Ikebuchi
  • J. Sawada
Original Research Papers


The effects of three Ca2+-ATPase inhibitors, thapsigargin (TG), cyclopiazonic acid (CPA), and 2,5-di(tert-butyl)-1,4-hydroquinone (DTBHQ), on the Ca2+ response, degranulation, and leukotriene C4 (LTC4) release in RBL-2H3 cells were investigated. All three compounds elevated the intracellular free Ca2+ concentration ([Ca2+]i), and caused degranulation in the presence of 12-O-tetradecanoylphorbol-13-acetate (TPA), a protein kinase C activator. The dose-dependency of each compound in the Ca2+ response was in good agreement with that in degranulation. TG and CPA also caused the release of LTC4 in a dose-dependent manner, and this effect was unaffected by TPA or calphostin C, a selective PKC inhibitor. DTBHQ, however, did not induce LTC4 release, and rather inhibited the antigen-induced release of LTC4. These results suggest [1] that both degranulation and LTC4 release caused by these compounds are dependent on their [Ca2+]i increasing effect, [2] that degranulation and LTC4 release are mediated via independent pathways following the Ca2+ response, and [3] that DTBHQ additionally prevents the synthesis of LTC4 possibly by inhibition of 5-lipoxygenase.

Key words

Basophilic leukemia cell Ca2+-ATPase inhibitor Cytosolic calcium level Degranulation Leukotriene C4 release 


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Copyright information

© Birkhäuser Verlag 1996

Authors and Affiliations

  • R. Akasaka
    • 1
  • R. Teshima
    • 1
  • H. Ikebuchi
    • 1
  • J. Sawada
    • 1
  1. 1.Division of Biochemistry and ImmunochemistryNational Institute of Health SciencesTokyoJapan

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