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A rapid separation of citrus carotenoids by thin-layer chromatography

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Summary

A rapid separation of citrus carotenoid pigments is achieved by successive thin-layer chromatographic separations on two different adsorbents. The first chromatography on silica gel G developed with the solvent system acetone-petroleum ether (30∶70) gives a preliminary fractionation into groups with different polarity (hydrocarbons, monols, diols and polyols). A further separation of each group into individual carotenoids is obtained by rechromatography on MgO-Kieselguhr (1∶1, w/w). The same solvent system is used, the acetone percentage being increased according to the polarity of each group (from 4% to 30%).

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Abbreviations

(A):

Acetone

(PE):

Petroleum ether

(BHT):

Butylated hydroxytoluene

References

  1. A. L. Curt, J. Agric. Food Chem.1, 456, (1953).

    Google Scholar 

  2. H. Yokoyama andC. E. Vandercook, J. Food Sci.32, 42 (1967).

    CAS  Google Scholar 

  3. H. Yokoyama andM. J. White, J. Agric. Food Chem.15, 693 (1967).

    Article  CAS  Google Scholar 

  4. J. Gross, M. Gabai andA. Lifshitz, J. Food Sci.36, 466 (1971).

    CAS  Google Scholar 

  5. J. Gross, M. Gabai andA. Lifshitz, Phytochemistry11, 303 (1972).

    CAS  Google Scholar 

  6. I. Stewart andT. A. Wheaton, J. Chromatogr.55, 325 (1971).

    Article  CAS  Google Scholar 

  7. I. Stewart, J. Assoc. Off. Anal. Chem.60, 132 (1977).

    CAS  Google Scholar 

  8. I. Stewart J. Agric. Food Chem.25, 1132 (1977).

    Article  CAS  Google Scholar 

  9. I. Stewart andT. A. Wheaton, Phytochemistry,12, 2947 (1973).

    Article  CAS  Google Scholar 

  10. J. Gross, A. Zahariae, F. Lenz andG. Eckhardt, Z. Pflanzenphysiol.89, 321 (1978).

    CAS  Google Scholar 

  11. G. Britton andT. W. Goodwin, in “Methods in Enzymology”, vol. XVIII,D. B. McCormick andL. D. Wright, eds., Academic Press, N.Y., 1971; p. 654.

    Google Scholar 

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Authors and Affiliations

  1. Institut für Obstbau und Gemüsebau der Universität Bonn, FRG

    Jeana Gross

  2. Department of Organic Chemistry, Laboratory of Natural Products, The Hebrew University of Jerusalem, Jerusalem, Israel

    Jeana Gross

Authors
  1. Jeana Gross
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Gross, J. A rapid separation of citrus carotenoids by thin-layer chromatography. Chromatographia 13, 572–573 (1980). https://doi.org/10.1007/BF02311862

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  • DOI: https://doi.org/10.1007/BF02311862

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