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Chromatographia

, Volume 21, Issue 10, pp 599–600 | Cite as

Direct fluorescence detection of non-steroidal anti-inflammatory agents separated by TLC with 9-isothiocyanatoacridine derivatives

  • C. Sârbu
  • C. Măruţoiu
  • M. Vlassa
Short Communication

Summary

Non-steroidal anti-inflammatory agents were separated by thin-layer chromatography, utilizing precoated plates with silica gel R as the stationary phase and chloroform:methanol:25% ammonia (80∶15∶5), as the mobile phase.

The spots were visualized by spraying with 0.1% solution of 9-isothiocyantoacridine derivatives in methylene chloride or benzene and irradiating with UV at 254 and 366nm. The visual detection limit of a spot was 0.1μg.

Key Words

Thin-layer chromatography Anti-inflammatory agents 9-Isothiocyanatoacridine derivatives Fluorescence detection 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1986

Authors and Affiliations

  • C. Sârbu
    • 1
  • C. Măruţoiu
    • 2
  • M. Vlassa
    • 2
  1. 1.Faculty of Mechanical EngineeringPolytechnic InstituteCluj-NapocaRomania
  2. 2.Institute of ChemistryCluj-NapocaRomania

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