Direct fluorescence detection of non-steroidal anti-inflammatory agents separated by TLC with 9-isothiocyanatoacridine derivatives
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Non-steroidal anti-inflammatory agents were separated by thin-layer chromatography, utilizing precoated plates with silica gel R as the stationary phase and chloroform:methanol:25% ammonia (80∶15∶5), as the mobile phase.
The spots were visualized by spraying with 0.1% solution of 9-isothiocyantoacridine derivatives in methylene chloride or benzene and irradiating with UV at 254 and 366nm. The visual detection limit of a spot was 0.1μg.
Key WordsThin-layer chromatography Anti-inflammatory agents 9-Isothiocyanatoacridine derivatives Fluorescence detection
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