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Determination of particulate fatty acids as P-bromophenacyl- or phenylphenacylesters using HPLC

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Summary

A method for the determination of fatty acids of the molecular weight range of propionic to erucic acid is reported. The acids are converted to either p-bromophenacyl-or p-phenylphenacylesters and the esters are separated by HPLC and detected by UV-absorption. Twentytwo esters, including saturated, monoolefinic and polyunsaturated ones were separated within one HPLC-run. The detection limit is about 5 pmol per fatty acid. The application of the method to particulate material is described.

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Osterroht, C. Determination of particulate fatty acids as P-bromophenacyl- or phenylphenacylesters using HPLC. Chromatographia 23, 419–422 (1987). https://doi.org/10.1007/BF02311817

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  • DOI: https://doi.org/10.1007/BF02311817

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