, Volume 6, Issue 5, pp 217–222 | Cite as

Temperature as a parameter in the anion exchange chromatography of hop bitter acids

  • R. Vanheertum


The chromatographic behaviour of nine hop bitter acids on anion exchangers was studied at temperatures between 30° and 60°C. Their retention times diminish with increasing temperature and there is a linear relationship between the logarithm of the adjusted retention time and the reciprocal absolute temperature. Some separation factors increase and others decrease as a function of temperature. The theoretical plate numbers of the columns go through maxima, located between 38° and 50°C. The resolution of the substance pairs decreases or increases in the temperature range studied and very pronounced resolution changes are found among the product pairs characterised by low separation factors.


Separation Factor Theoretical Plate Number Reciprocal Absolute Temperature Substance Pair Adjusted Retention Time 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. [1]
    Dybscynski, R., J. Chromatogr.31, 155 (1967).CrossRefGoogle Scholar
  2. [2]
    Lee, K. S., Samuelson, O., Anal. Chim. Acta37, 359 (1967).CrossRefGoogle Scholar
  3. [3]
    Bengtsson, L., Samuelson, O., Chromatographia4, 142 (1971).CrossRefGoogle Scholar
  4. [4]
    Horvath, C. G., Preiss, B. A., Lipsky, S. R., Anal. Chem.39, 1422 (1967).CrossRefGoogle Scholar
  5. [5]
    Ashurst, P. R. J. Inst. Brew.72, 561 (1966).CrossRefGoogle Scholar
  6. [6]
    Ashurst, P. R., J. Inst. Brew.74, 157 (1968).Google Scholar
  7. [7]
    Hamilton, P. B., Anal. Chem.30, 915 (1958).Google Scholar
  8. [8]
    Sie, S. T., Van Den Hoed, N., J. Chromatogr. Sci.7, 257 (1969).CrossRefGoogle Scholar
  9. [9]
    Tomihito Kambara, Hiroshi Kokama, J. Chromatogr.17, 66 (1965).CrossRefGoogle Scholar
  10. [10]
    De Wet, W. J., Pretorius, V., Anal. Chem.30, 325 (1958).CrossRefGoogle Scholar
  11. [11]
    Parissakis, G., Vranti-Piscou, D., Kontoyannakos, J., Chromatographia3, 541 (1970).CrossRefGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn GmbH 1973

Authors and Affiliations

  • R. Vanheertum
    • 1
  1. 1.Laboratory of Organic ChemistryUniversity of GentGentBelgium

Personalised recommendations