Temperature as a parameter in the anion exchange chromatography of hop bitter acids
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The chromatographic behaviour of nine hop bitter acids on anion exchangers was studied at temperatures between 30° and 60°C. Their retention times diminish with increasing temperature and there is a linear relationship between the logarithm of the adjusted retention time and the reciprocal absolute temperature. Some separation factors increase and others decrease as a function of temperature. The theoretical plate numbers of the columns go through maxima, located between 38° and 50°C. The resolution of the substance pairs decreases or increases in the temperature range studied and very pronounced resolution changes are found among the product pairs characterised by low separation factors.
KeywordsSeparation Factor Theoretical Plate Number Reciprocal Absolute Temperature Substance Pair Adjusted Retention Time
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