Chromatographia

, Volume 23, Issue 3, pp 207–208 | Cite as

Effect of halides on the TLC resolution of amino acids below their isoelectric points

  • R. Bhushan
  • Imran Ali
Short Communications

Abstract

TLC of a fifteen component mixture of amino acids has been carried out in two ways; firstly, the amino acids were treated with halides below their isoelectric points and chromatographed on plain silica plates, and secondly the amino acids in their cationic forms were chromatographed on silica plates impregnated with halides, keeping the same solvent system. The resolution is considered to be affected by hydrophobic interactions between silica gel and amino acid molecule and by the polarity and the flow of the mobile phase. The method provides resolution of 10–11 amino acids from the fifteen component mixture.

Key Words

Thin-layer chromatography Halide ion impregnated silica gel Amino acids 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1987

Authors and Affiliations

  • R. Bhushan
    • 1
  • Imran Ali
    • 1
  1. 1.Department of ChemistryUniversity of RoorkeeRoorkeeIndia

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