Skip to main content
SpringerLink
Log in

Enzymatic hydrolysis of pectic substances by gel-permeation chromatography

  • Originals
  • Published:
Chromatographia Aims and scope Submit manuscript

Summary

A rapid liquid chromatographic (HPLC) method for the determination of pectinolytic activity of enzymes produced by Aspergillus Niger and Rhizopus species is reported.

Compared with more conventional methods, HPLC is more reliable and has a much improved maximum sensitivity. The limit of quantitation of galacturonic acid is 0.1μg.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. W. M. Fogarty, O. P. Ward, Process Biochem.,13, 17 (1972).

    Google Scholar 

  2. A. Lopez, L. I. Li-Hsieng, Fed. Technol.22, 1023 (1968).

    CAS  Google Scholar 

  3. W. V. Cruess, J. Besone, Fruit. Prod. J.20, 365 (1941).

    Google Scholar 

  4. M. Somogyi, J. Biol. Chem.,195, 19 (1952).

    CAS  Google Scholar 

  5. P. J. Mill, R. Tuttobello, Biochem. J.79, 57 (1961).

    CAS  Google Scholar 

  6. N. Blumenkrantz, G. Asboe-Hansen, Anal. Bioch.54, 484 (1973).

    CAS  Google Scholar 

  7. E. F. Jansen, L. R. MacDonnell, Arch. Biochem.8, 97 (1945).

    CAS  Google Scholar 

  8. H. Deuel, F. Weber, Helv. Chim. Acta,30, 1523 (1947).

    CAS  Google Scholar 

  9. J. D. MacMillan, H. J. Phaffond, R. H. Waughn, Biochem.3, 572 (1964).

    CAS  Google Scholar 

  10. O. Mikes, J. Sedlacková, L. Rexova, J. Omelková, J. Chromatogr.,207, 99 (1981).

    Article  CAS  Google Scholar 

  11. B. S. Luh, H. Y. Phaff, Arch. Bioch. and Biophys.19, 102 (1953).

    Google Scholar 

  12. Lineweaver, F. E. Jansen, Adv. Enzymol.,11, 267 (1951).

    CAS  Google Scholar 

  13. H. Deuel, E. Stutz, Adv. Enzymol.,20, 341 (1958).

    CAS  Google Scholar 

  14. P. D. English, A. Maglothin, K. Keegstra, P. Albersheim, Plant. Phisiol.49, 293 (1972).

    CAS  Google Scholar 

  15. J. H. Phaff, Methods Enzymol.8, 636 (1966).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dipartimento di Chimica, Universita degli Studi “La Sapienza”, P. le Aldo Moro 5, 00185, Rome, Italy

    V. Carunchio, A. M. Girelli & A. M. Tarola

  2. Istituto di Cromatografia del CNR, Area della Ricerca di Roma, Monterotondo Stazione, C.P. 10, 00016, Roma, Italy

    M. Sinibaldi

Authors
  1. V. Carunchio
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. M. Girelli
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Sinibaldi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. M. Tarola
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Carunchio, V., Girelli, A.M., Sinibaldi, M. et al. Enzymatic hydrolysis of pectic substances by gel-permeation chromatography. Chromatographia 25, 870–874 (1988). https://doi.org/10.1007/BF02311420

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02311420

Key Words

Search

Navigation