Summary
Using a camomile flavonoid extract as the sample and four different reversed-phase partition systems, the ability of the Simplex procedure to produce optimum gradient separation of unknown multicomponent mixtures was checked against the linear solvent strength (LSS) gradient elution theory. On the same partition systems the mean solvent strenghts were measured by experimentally determined logk′ vs. mobile phase composition plots. These mean solvent strengths are compared to those inferred from the optimum gradients and the usefulness of LSS theory in multicomponent mixture gradient elution optimization is discussed.
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Dadicated to Prof. Dr. A Liberti on the occasion of his 70th birthday.
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Dondi, F., Kahie, Y.D., Lodi, G. et al. Comparison of the sequential simplex method and linear solvent strength theory in HPLC gradient elution optimization of multicomponent flavonoid mixtures. Chromatographia 23, 844–849 (1987). https://doi.org/10.1007/BF02311411
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DOI: https://doi.org/10.1007/BF02311411