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An efficient procedure for extraction and determination of steroids in the tissue of laboratory animals

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Summary

A high-performance liquid chromatographic method for analysis of steroids in the tissue of laboratory animals is described. A two-stage solid extraction procedure, utilizing Sep-pak C18 and graphitized carbon black, gives a purified product. Steroids in this extract were separated by multistep gradient elution with a reversedphase liquid chromatography system, using acetonitrilewater as solvent. Several columns with different geometry and phase loading were investigated. Optimum resolution was obtained with a medium covered and spherical particle.

The applicability of this method was successfully demonstrated in the analysis of the testis and adrenal gland of rat.

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Authors and Affiliations

  1. Dipartimento di Chimica, Universitá “La Sapienza” di Roma, Piazza Aldo Moro, 00185, Roma, Italy

    A. Laganà, G. d'Ascenzo & A. Marino

Authors
  1. A. Laganà
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  2. G. d'Ascenzo
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  3. A. Marino
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Dedicated to Prof. Dr. A. Liberti on the occasion of his 70th birthday.

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Laganà, A., d'Ascenzo, G. & Marino, A. An efficient procedure for extraction and determination of steroids in the tissue of laboratory animals. Chromatographia 23, 796–802 (1987). https://doi.org/10.1007/BF02311402

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  • DOI: https://doi.org/10.1007/BF02311402

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