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Nutritional requirements and growth characteristics of a biosurfactant-producingRhodococcus bacterium

  • A. S. Abu-Ruwaida
  • I. M. Banat
  • S. Haditirto
  • A. Khamis
Research Papers

Abstract

The nutritional requirements and growth characteristics of a biosurfactant-producingRhodococcus bacterium isolated from Kuwaiti soil were determined. Maximum cell yields (6.6 g/l) and biosurfactant production were achieved with a medium containing 2% (v/v)n-paraffin as a carbon and energy source, 0.2% lactose broth, optimal concentrations of nitrogen (nitrate), phosphorus, iron, magnesium and sodium sources, and minimal concentrations of potassium and trace element sources. The optimal pH was 6.8 for surfactant production and optimal temperature was 37°C. The biosurfactant produced after 16 to 33 h growth in a 7 I fermenter decreased both surface tension and interfacial tension of culture broth to below 27 and 1.8 mN/m, respectively, and was effective at critical micelle dilutions of 10−3. Data on biosurfactant biosynthesis suggest that the product is produced as a primary metabolite and, therefore, could be produced effectively under continuous fermentation conditions.

Keywords

Surfactant Fermentation Surface Tension Lactose Interfacial Tension 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Rapid Communications of Oxford Ltd 1991

Authors and Affiliations

  • A. S. Abu-Ruwaida
  • I. M. Banat
  • S. Haditirto
  • A. Khamis

There are no affiliations available

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