Reversed-phase high-performance thin-layer chromatography of hematoporphyrin and its nickel, copper and zinc complexes
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The thin-layer chromatographic mobility of hematoporphyrin IX (Hp) and its metal complexes on octyl (C8)- or octadecyl (C18)-bonded silica gel plate is described. The mobility order for those compounds is regular on both kinds of plate with various compositions of methanol-phosphate buffer mixtures as the developing solvents; thus, Cu-complex < Ni-complex < Hp (free acid) < Zn-complex. The combination of a C18-plate with an 85∶15 (vol/vol) mixture of methanol and phosphate buffer (pH 3) is recommended for the successful separation of these four compounds.
Key WordsThin-layer chromatography Porphyrin Metalloporphyrins Hematoporphyrin IX
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- M. Kobayashi, K. Saitoh, N. Suzuki, Chromatographia,20, 49 (1985).Google Scholar
- C. J. Gomer, T. J. Dougherty, Cancer Res.,39, 146 (1979).Google Scholar
- D. Kessel, T.-H. Chou, Cancer Res.,43, 1994 (1983).Google Scholar
- C. J. Gomer, D. R. Doiron, J. V. Jester, B. C. Szirth, A. L. Murphree, Cancer Res.,43, 721 (1983).Google Scholar