Chromatographia

, Volume 23, Issue 1, pp 43–46 | Cite as

Identification of the components of aldoses in a tautomeric equilibrium mixture as their trimethylsilyl ethers by capillary gas chromatography

  • M. Paez
  • I. Martínez-Castro
  • J. Sanz
  • A. Olano
  • A. Garcia-Raso
  • F. Saura-Calixto
Originals

Abstract

Equilibrium tautomers of aldopentoses (arabinose, ribose, xylose and lyxose) and aldohexones (allose, altrose, gulose, idose and talose) have been analyzed as their trimethyl-silyl ethers by capillary GC. Pentoses were separated on SE-54 and hexoses on OV-225. The four components of the tautomer equilibrium mixture have been identified using GC/MS and NMR data.

Key Words

Gas chromatography Carbohydrate TMS-ethers Tautomeric equilibrium mixtures of aldoses MS and NMR identification 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    S. J. Angyal, Adv. Carbohyd. Chem. & Biochem.42, 15 (1984).Google Scholar
  2. [2]
    C. C. Sweeley, R. Bentley, M. Makita, W. W. Wells, J. Am. Chem. Soc.85, 2497 (1963).CrossRefGoogle Scholar
  3. [3]
    L. Mester, H. S. El-Khadem in “The Carbohydrates” Ed.W. Pigman andW. Horton, Academic Press, London (1980) 11B.Google Scholar
  4. [4]
    A. G. W. Bradbury, D. J. Halliday, D. J. Medcalf, J. Chromatogr.213, 146 (1981).CrossRefGoogle Scholar
  5. [5]
    M. I. El-Hazmi, K. R. Stauffer, C. Olney, Chromatographia7, 403 (1985).Google Scholar
  6. [6]
    K. Tesarik, J. Chromatogr.65, 295 (1971).Google Scholar
  7. [7]
    T. E. Acree, R. S. Shallenberger, L. R. Mattick, Carbohyd. Res.6, 498 (1968).Google Scholar
  8. [8]
    T. E. Acree, R. S. Shallenberger, C. Y. Lee, J. W. Einset, Carbohyd. Res.10, 355 (1969).Google Scholar
  9. [9]
    L. E. Vidaurreta, L. B. Fournier, M. L. Burks, Anal. Chim. Acta52, 507 (1970).CrossRefGoogle Scholar
  10. [10]
    H. Ch. Curtius, M. Muller, J. A. Vollmin, J. Chromatogr.37, 216 (1968).CrossRefGoogle Scholar
  11. [11]
    I. Martínez-Castro, A. Olano, M. Juárez, F. Morais, Proc. III Congr. Nac. Quim. EFCE Pub. Series2, 153 (1980).Google Scholar
  12. [12]
    J. Longren, S. Svensson, Adv. Carbohyd. Chem. Biochem.29, 42 (1974).Google Scholar
  13. [13]
    T. Radford, D. C. Dejongh, in “Biochem. Applications of Mass Spectrometry” Ed.G. R. Waller, Wiley-Interscience, New-York (1980).Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1987

Authors and Affiliations

  • M. Paez
    • 1
  • I. Martínez-Castro
    • 1
  • J. Sanz
    • 1
  • A. Olano
    • 2
  • A. Garcia-Raso
    • 3
  • F. Saura-Calixto
    • 3
  1. 1. Instituto de Química Organica GeneralMadridSpain
  2. 2.Instituto de Fermentaciones Industriales (CSIC)MadridSpain
  3. 3.Departamento de Química Organica, Facultad de CienciasUniversidad de las Islas BalearesSpain

Personalised recommendations