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Antonie van Leeuwenhoek

, Volume 51, Issue 2, pp 203–218 | Cite as

Plasmid-determined cloacin DF13-susceptibility inEnterobacter cloacae andKlebsiella edwardsii; identification of the cloacin DF13/aerobactin outer membrane receptor proteins

  • Willy J. A. Krone
  • Gregory Koningstein
  • Frits K. de Graaf
  • Bauke Oudega
Genetics and Molecular Biology

Abstract

BothEnterobacter cloacae H478 andKlebsiella edwardsii S15 were shown to harbour a relatively large conjugative plasmid that coded for cloacin DF13-susceptibility and the production and uptake of a hydroxamate iron chelator, most probably aerobactin. Protein-blotting experiments with antiserum raised against the purified cloacin DF13/aerobactin receptor protein fromEscherichia coli (Co1V-K30) revealed that the corresponding outer membrane receptor proteins ofEnt. cloacae H478 andK. edwardsii S15 had apparent mol wts of 85 000 and 76000, respectively.E. coli transconjugants harbouring either the plasmid fromEnt. cloacae H478 orK. edwardsii S15 expressed a cloacin DF13/aerobactin outer membrane receptor protein with a mol wt of 74000. The receptor protein encoded by theEnt. cloacae andK. edwardsii plasmids were immunologically more related to each other than to the pCo1V-K30-encoded receptor protein.

Keywords

Iron Outer Membrane Receptor Protein Membrane Receptor Iron Chelator 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Kluwer Academic Publishers 1985

Authors and Affiliations

  • Willy J. A. Krone
    • 1
  • Gregory Koningstein
    • 1
  • Frits K. de Graaf
    • 1
  • Bauke Oudega
    • 1
  1. 1.Department of Microbiology, Biological LaboratoryVrije UniversiteitAmsterdamThe Netherlands

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