Antonie van Leeuwenhoek

, Volume 55, Issue 1, pp 67–82 | Cite as

Influence of environmental parameters on polyphosphate accumulation inAcinetobacter sp.

  • Johan W. Van Groenestijn
  • Martina Zuidema
  • Jacques J. M. Van De Worp
  • Maria H. Deinema
  • Alexander J. B. Zehnder
General Papers


The regulation of and the optimum conditions for polyphosphate accumulation inAcinetobacter sp. were determined.Acinetobacter strain 210A accumulated polyphosphate in the presence of an intra- or extracellular energy source. The accumulation of polyphosphate during endogenous respiration was stimulated by streptomycin and inhibited by KCN. The highest amount of polyphosphate was found in cells in which energy supply was not limited, namely at low growth rates under sulphur limitation, and in the stationary phase of growth when either the nitrogen or the sulphur source was depleted. The phosphorus accumulation was not affected by the pH between 6.5 and 9. There was a pronounced effect of the temperature on phosphorus accumulation but is varied from strain to strain.Acinetobacter strain 210A accumulated more phosphate at low temperatures, strain B8 showed an optimum accumulation at 27.5° C, while strain P accumulated phosphorus independently of the temperature. The optimum temperature for growth ofAcinetobacter strains tested ranged from 25 to 33° C, and the optimum pH was between 6 and 9.

Key words

biological phosphate removal activated sludge nutrient limitation energy reserve Acinetobacter 


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Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • Johan W. Van Groenestijn
    • 1
  • Martina Zuidema
    • 1
  • Jacques J. M. Van De Worp
    • 1
  • Maria H. Deinema
    • 1
  • Alexander J. B. Zehnder
    • 1
  1. 1.Department of MicrobiologyAgricultural UniversityWageningenThe Netherlands

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