Micropropagation ofPrunus mume
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Shoot proliferation from axillary buds ofPrunus mume Sieb. et Zucc. was obtained on Woody Plant Medium (WPM) supplemented with 1 to 5 μM benzyladenine, 3% sorbitol and solidified with 0.5 to 0.7% agar. Effects of different carbon sources on shoot proliferation were examined. Glucose provided better shoot proliferation than sucrose, sorbitol and fructose. In the presence of sucrose, leaf chlorosis occurred and shoots gradually declined. Best rooting percentage was obtained on WPM supplemented with 1 μM naphthaleneacetic acid. Rooted plantlets were acclimatized under intermittent mist. However, survival rate was relatively low (20 to 30%).
Key wordsfructose in vitro rooting shoot proliferation sorbitol
Murashige and Skoog (1962)
Woody Plant Medium [Lloyd and McCown (1981)]
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