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Plant Cell, Tissue and Organ Culture

, Volume 46, Issue 3, pp 265–267 | Cite as

Micropropagation ofPrunus mume

  • Hisashi Harada
  • Yasuhiro Murai
Research Note

Abstract

Shoot proliferation from axillary buds ofPrunus mume Sieb. et Zucc. was obtained on Woody Plant Medium (WPM) supplemented with 1 to 5 μM benzyladenine, 3% sorbitol and solidified with 0.5 to 0.7% agar. Effects of different carbon sources on shoot proliferation were examined. Glucose provided better shoot proliferation than sucrose, sorbitol and fructose. In the presence of sucrose, leaf chlorosis occurred and shoots gradually declined. Best rooting percentage was obtained on WPM supplemented with 1 μM naphthaleneacetic acid. Rooted plantlets were acclimatized under intermittent mist. However, survival rate was relatively low (20 to 30%).

Key words

fructose in vitro rooting shoot proliferation sorbitol 

Abbreviations

BA

benzyladenine

IBA

indolebutyric acid

MS

Murashige and Skoog (1962)

NAA

naphthaleneacetic acid

WPM

Woody Plant Medium [Lloyd and McCown (1981)]

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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Hisashi Harada
    • 1
  • Yasuhiro Murai
    • 1
  1. 1.Faculty of AgricultureShizuoka UniversityShizuokaJapan

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